| A method for the scrutiny of live mammalian cells in culture and for the measurement of their proliferative ability after x-irradiation. | |
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MedLine Citation:
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PMID: 7014493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method is described for the observation of live mammalian cells in culture with an incubated phase-contrast microscope. A sample of plated cells may be watched and their respective capacities to form a colony measured by daily cell counts. The method has first been used to make direct estimations of the plating efficiency of the diploid line of Syrian hamster fibroblasts, BHK 21 C13, and then to observe the response of synchronous samples of these cells to 220 kV X-rays. A dose of 1.4 Gy given in Gl has no immediate detectable effect on cell or unclear morphology, and cell capacity to reach post-irradiation mitosis in unimpaired apart from delay. In contrast, after this mitosis is completed, descendant cells from some mitoses retain a normal form and clonogenic capacity, whereas the cells from other mitoses show varying degrees of abnormality and produce either slow-growth or stop-growth (micro-) colonies. |
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Authors:
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S J Grote; G P Joshi; S H Revell; C A Shaw |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation biology and related studies in physics, chemistry, and medicine Volume: 39 ISSN: 0020-7616 ISO Abbreviation: Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. Publication Date: 1981 Apr |
Date Detail:
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Created Date: 1981-07-20 Completed Date: 1981-07-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374725 Medline TA: Int J Radiat Biol Relat Stud Phys Chem Med Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 377-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / radiation effects* Cells, Cultured / radiation effects* Cricetinae Cytological Techniques Fibroblasts / radiation effects Mesocricetus Time Factors X-Rays |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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