Document Detail


A method for the scrutiny of live mammalian cells in culture and for the measurement of their proliferative ability after x-irradiation.
MedLine Citation:
PMID:  7014493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described for the observation of live mammalian cells in culture with an incubated phase-contrast microscope. A sample of plated cells may be watched and their respective capacities to form a colony measured by daily cell counts. The method has first been used to make direct estimations of the plating efficiency of the diploid line of Syrian hamster fibroblasts, BHK 21 C13, and then to observe the response of synchronous samples of these cells to 220 kV X-rays. A dose of 1.4 Gy given in Gl has no immediate detectable effect on cell or unclear morphology, and cell capacity to reach post-irradiation mitosis in unimpaired apart from delay. In contrast, after this mitosis is completed, descendant cells from some mitoses retain a normal form and clonogenic capacity, whereas the cells from other mitoses show varying degrees of abnormality and produce either slow-growth or stop-growth (micro-) colonies.
Authors:
S J Grote; G P Joshi; S H Revell; C A Shaw
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology and related studies in physics, chemistry, and medicine     Volume:  39     ISSN:  0020-7616     ISO Abbreviation:  Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-07-20     Completed Date:  1981-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374725     Medline TA:  Int J Radiat Biol Relat Stud Phys Chem Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  377-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / radiation effects*
Cells, Cultured / radiation effects*
Cricetinae
Cytological Techniques
Fibroblasts / radiation effects
Mesocricetus
Time Factors
X-Rays

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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