Document Detail

A method for quantitative wet chemical analysis of urinary calculi.
MedLine Citation:
PMID:  6086179     Owner:  NLM     Status:  MEDLINE    
We describe a simple method for quantitative chemical analysis of urinary calculi requiring no specialized equipment. Pulverized calculi are dried over silica gel at room temperature and dissolved in nitric acid, which was the only effective agent for complete dissolution. Calcium, magnesium, ammonium, and phosphate are then determined by conventional methods. Oxalate is determined by a method based on the quenching action of oxalate on the fluorescence of a zirconium-flavonol complex. Uric acid, when treated with nitric acid, is stoichiometrically converted to alloxan, which is determined fluorimetrically with 1,2-phenylenediamine. Similarly, cystine is oxidized by nitric acid to sulfate, which is determined turbidimetrically as barium sulfate. Protein is determined spectrophotometrically as xanthoprotein. The total mass recovery of authentic calculi was 92.2 +/- 6.7 (SD) per cent. The method permits analysis of calculi as small as 1.0 mg. Internal quality control is performed with specially designed control samples.
L Larsson; B Sörbo; H G Tiselius; S Ohman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  140     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-09-19     Completed Date:  1984-09-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  9-20     Citation Subset:  IM    
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MeSH Terms
Cations / analysis
Chemistry Techniques, Analytical / methods
Cystine / analysis
Nitric Acid
Oxalates / analysis
Phosphates / analysis
Proteins / analysis
Silicon Dioxide
Uric Acid / analysis
Urinary Calculi / etiology,  metabolism*,  therapy
Reg. No./Substance:
0/Cations; 0/Nitrates; 0/Oxalates; 0/Phosphates; 0/Proteins; 56-89-3/Cystine; 60650-90-0/silochrome; 69-93-2/Uric Acid; 7631-86-9/Silicon Dioxide; 7697-37-2/Nitric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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