| A method for preparing whole-body sections suitable for autoradiographic, histological and histochemical studies. | |
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MedLine Citation:
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PMID: 3523839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for the preparation of whole-body sections suitable for autoradiographic and histochemical study is described. Radioactive calcium chloride or [14C]proline was injected into the abdominal cavity of a rat. Thirty-five minutes after injection of calcium chloride or 40 min after injection of proline the rat was frozen in a mixture of hexane and solid carbon dioxide and blocked in 5% sodium carboxymethyl cellulose. The carboxymethyl cellulose block was trimmed and a piece of copy paper was attached to the surface of the block with cellulose tape. Cryotome sections cut from the block were transferred from the paper to a glass slide coated with synthetic rubber adhesive. For whole-body autoradiography, sections were freeze-dried for 2 days and then placed against X-ray film. For light microscopic autoradiography, the freeze-dried sections were covered with a dried film of photographic emulsion. For histochemical use, the sections were fixed by raising the temperature to 4 C after immersion in 100% ethanol below -10 C. For histological observation, sections were postfixed with 2.5% glutaraldehyde and stained. Whole-body and light microscopic autoradiographs showed that sections so prepared could be used for the demonstration of soluble substances in whole-body sections and for detailed autoradiography at the light microscopic level, and the stained sections could be used for histological and histochemical studies. |
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Authors:
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T Kawamoto; M Shimizu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Stain technology Volume: 61 ISSN: 0038-9153 ISO Abbreviation: Stain Technol Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-08-11 Completed Date: 1986-08-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404535 Medline TA: Stain Technol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 169-83 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Calcium Chloride Freeze Drying Frozen Sections Histocytochemistry Histological Techniques* Microtomy / methods* Proline Rats |
| Chemical | |
Reg. No./Substance:
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10043-52-4/Calcium Chloride; 147-85-3/Proline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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