| A method for measuring free-nucleotide pool sizes and incorporation of labeled precursors: its application in studying myocardial pyrimidine nucleotides. | |
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MedLine Citation:
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PMID: 6625155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A procedure for a rapid and accurate determination of nucleotide pool sizes in heart muscle is described. The method involves an enzymatic cleavage of all nucleotides by phosphodiesterase to nucleoside 5'-monophosphates and an HPLC separation (Partisil 10 SAX) by isocratic or two-step elution. This method permits reproducible measurements of the pools of pyrimidine nucleotides which are particularly small in cardiac tissue. Moreover, this technique may be conveniently applied in studies on the incorporation of labeled precursors into free nucleotides. Experimental evidence is presented showing the accuracy of the method. |
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Authors:
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M Verdys; J Aussedat; J Olivares; A Ray; A Rossi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 132 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1983-11-25 Completed Date: 1983-11-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 137-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Monophosphate
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isolation & purification Animals Chromatography, High Pressure Liquid Myocardium / metabolism* Pyrimidine Nucleotides / isolation & purification, metabolism* Rats Uridine Monophosphate / isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Pyrimidine Nucleotides; 58-97-9/Uridine Monophosphate; 61-19-8/Adenosine Monophosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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