Document Detail


A method for the massive separation of highly purified, adult porcine islets of Langerhans.
MedLine Citation:
PMID:  2105435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the massive and reproducible isolation of highly purified, adult porcine islets of Langerhans is described. The successful combination of donor animal-strain selection with original procedures for pancreas retrieval and enzymatic digestion permitted us to separate uniquely massive concentrations of pure porcine islets with no need for mechanical disruption of the pancreatic tissue. Following our procedure, porcine islets, which fully retain viability and function, can be harvested easily and rapidly. Xenotransplantation of such islets, immunoprotected within algin/polyaminoacidic microcapsules, was associated with complete reversal of hyperglycemia in rodents with either spontaneous or streptozotocin-induced diabetes mellitus.
Authors:
R Calafiore; F Calcinaro; G Basta; M Pietropaolo; A Falorni; M Piermattei; P Brunetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  39     ISSN:  0026-0495     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-02-26     Completed Date:  1990-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  175-81     Citation Subset:  IM    
Affiliation:
Istituto di Patologia Speciale Medica e Metodologia Clinica, University of Perugia, School of Medicine, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / analysis
Culture Techniques
Diabetes Mellitus, Experimental / surgery
Hydrolysis
Hyperglycemia / surgery
Insulin / analysis
Islets of Langerhans* / analysis,  ultrastructure
Islets of Langerhans Transplantation
Methods
Mice
Peritoneum
Rats
Swine
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Blood Glucose; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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