| A method for the making and utility of gadolinium-labeled albumin microbubbles. | |
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MedLine Citation:
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PMID: 22056512 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: Perfluorocarbon-exposed sonicated dextrose albumin microbubbles (PESDA) binds scavenger receptors and can be noninvasively imaged. To enhance imaging, gadolinium (Gd)-labeled PESDA was developed and tested in a model of vascular inflammation by magnetic resonance imaging (MRI). METHODS AND RESULTS: Purified human serum albumin (HSA) (5%) was labeled with Gd via the covalent binding of diethylenetriaminepentacetic acid. Abdominal aortic tissues in Sprague-Dawley rats (n=5 per group) were analyzed by 7-T MRI and scanning electron microscopy to evaluate PESDA binding. Labeling-purified 5% human albumin resulted in an average of 16.1 Gd atoms per albumin molecule as determined by atomic absorption. Forty-eight hours after balloon angioplasty, aortic tissue was enhanced with Gd-PESDA as compared to control tissue. 7-T MRI of explanted tissues was sensitive to the detection of retained PESDA. Enhancement of aortic tissue in vivo was present albeit to a lesser extent than explanted tissue from the same animals. CONCLUSIONS: HSA was successfully labeled, and an albumin-based microbubble with Gd was synthesized. This contrast agent, Gd-PESDA, may serve as an additional agent for the MRI evaluation of innate inflammation and used to noninvasively image early vascular pathophysiologic processes. CONDENSED ABSTRACT: In this study, Gd-PESDA microbubbles and were synthesized and shown to detect the binding of these microbubbles using MRI in injured aortic tissue. The method for synthesizing Gd-PESDA is detailed, and the proposed utility of this new contrast agent is discussed. |
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Authors:
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Daniel R Anderson; Michael J Duryee; Robert P Garvin; Michael D Boska; Geoffrey M Thiele; Lynell W Klassen |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-4 |
Journal Detail:
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Title: Magnetic resonance imaging Volume: - ISSN: 1873-5894 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8214883 Medline TA: Magn Reson Imaging Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Experimental Immunology, Research in Cardiovascular Disease Laboratory at the University of Nebraska Medical Center, Division of Cardiology, Department of Internal Medicine, 982265 Nebraska Medical Center, Omaha, NE 68198-2265, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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