Document Detail


A method for making ribbons of semithin plastic sections.
MedLine Citation:
PMID:  7303013     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epoxy resin sections form strong, heat resistant ribbons if, prior to sectioning, contact cement has been painted onto the leading and trailing faces of the block. The forming ribbon floats onto a drop of water held in place by a wax line drawn across the back of the glass knife parallel to the cutting edge. A long trough made from stainless steel tubing is inserted horizontally into the drop, and as the ribbon lengthens it is directed into the trough. The ribbon can be carried in the trough to a hot plate for expansion and then poured onto a slide for mounting. The serial ribbons obtainable by this simple procedure greatly facilitate three dimensional reconstruction of fine tissue structures.
Authors:
R D Campbell
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Stain technology     Volume:  56     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1981 Jul 
Date Detail:
Created Date:  1982-01-20     Completed Date:  1982-01-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  247-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adhesives
Microtomy / instrumentation,  methods*
Grant Support
ID/Acronym/Agency:
NS 12446/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Adhesives

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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