| A method for the isolation and purification of mouse peripheral blood monocytes. | |
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MedLine Citation:
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PMID: 20457160 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based on immuno-magnetic labeling. By negative selection we were able to isolate enriched fractions (>50% purity) of unlabeled monocytes. A subsequent positive selection step resulted in purities of > or =90% viable and functional monocytes. This method is of general use for studying mouse monocyte biology. |
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Authors:
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Erica Houthuys; Kiavash Movahedi; Patrick De Baetselier; Jo A Van Ginderachter; Peter Brouckaert |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-08 |
Journal Detail:
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Title: Journal of immunological methods Volume: 359 ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Copyright Information:
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2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department for Molecular Biomedical Research, VIB, Ghent, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / immunology Cells, Cultured Female Flow Cytometry Immunomagnetic Separation / methods* Leukocytes, Mononuclear / cytology*, immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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