Document Detail


A method for the intracranial delivery of reagents to voltammetric recording sites.
MedLine Citation:
PMID:  22580054     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carbon fiber microelectrodes are widely used for electrochemical monitoring in the intact brain. The local delivery of reagents to the recording site is often desirable. The approach of co-implanting a micropipette near the microelectrode presents some limitations that are overcome by the use of double-barreled devices. One barrel supports the carbon fiber and the other barrel serves as a pipet for local reagent delivery. Some studies have used iontophoretic delivery but here we consider the alternative approach of pressure ejection. However, placing the pipet so close to the electrode raises the risk that reagent can leak into the recording site. This problem is easily solved. We filled the tip of the pipet with vehicle solution, the barrel with a reagent solution, and separated the two solutions with an air gap to prevent their mixing. With this approach, reagent is delivered only after 'priming' pressure pulses: we show in two examples that unintended reagent delivery (leakage) prior to the priming pulses is non-detectable.
Authors:
Keith F Moquin; Andrea Jaquins-Gerstl; Adrian C Michael
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-09
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  208     ISSN:  1872-678X     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2013-05-22     Revised Date:  2013-07-17    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon / chemistry
Drug Delivery Systems / instrumentation*
Electrophysiology / instrumentation*,  methods
Male
Microelectrodes / standards*
Microinjections / instrumentation*,  methods
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
MH075989/MH/NIMH NIH HHS; R01 MH075989/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/carbon fiber; 7440-44-0/Carbon
Comments/Corrections

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