Document Detail


A method for freezing synchronous mitotic and G1 cells.
MedLine Citation:
PMID:  3595736     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modification of the protocol developed by Kawamoto, J C & Barrett, J N, Brain res (1986), in press for freezing primary neuron cultures in a solution containing low sodium and high lactate and potassium concentrations was used to freeze synchronous mitotic and G1 CHO cells. After thawing, the cells behaved as if they had never been frozen with respect to cell growth, cell division, plating efficiency, and hyperthermic sensitivity.
Authors:
M J Borrelli; M A Mackey; W C Dewey
Related Documents :
22350946 - Cardiac telocytes - their junctions and functional implications.
21562656 - Magnetoresistive chip cytometer.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  170     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-07-29     Completed Date:  1987-07-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  363-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle*
Cell Line
Cell Survival
Cricetinae
Freezing
Mitosis*
Preservation, Biological / methods*
Grant Support
ID/Acronym/Agency:
CA 09215/CA/NCI NIH HHS; CA 31813/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Modulation of intracellular glutathione concentrations alters lymphocyte activation and proliferatio...
Next Document:  A role for sulfated polysaccharide recognition in sponge cell aggregation.