| A method for freezing synchronous mitotic and G1 cells. | |
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MedLine Citation:
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PMID: 3595736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A modification of the protocol developed by Kawamoto, J C & Barrett, J N, Brain res (1986), in press for freezing primary neuron cultures in a solution containing low sodium and high lactate and potassium concentrations was used to freeze synchronous mitotic and G1 CHO cells. After thawing, the cells behaved as if they had never been frozen with respect to cell growth, cell division, plating efficiency, and hyperthermic sensitivity. |
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Authors:
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M J Borrelli; M A Mackey; W C Dewey |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 170 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1987 Jun |
Date Detail:
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Created Date: 1987-07-29 Completed Date: 1987-07-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 363-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Line Cell Survival Cricetinae Freezing Mitosis* Preservation, Biological / methods* |
| Grant Support | |
ID/Acronym/Agency:
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CA 09215/CA/NCI NIH HHS; CA 31813/CA/NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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