Document Detail

A method for estimating the nature and relative proportions of amorphous, single, and double-helical components in starch granules by (13)C CP/MAS NMR.
MedLine Citation:
PMID:  17266368     Owner:  NLM     Status:  MEDLINE    
An improved method to analyze the (13)C NMR spectra of native starches, which considers the contribution of the V-type conformation and the nature of the amorphous component, has been developed. Starch spectra are separated into amorphous and ordered subspectra, using intensity at 84 ppm as a reference point. The ordered subspectra of high amylose starches show the presence of both V-type single helices and B-type double helices. Relative proportions of amorphous, single, and double-helical conformations are estimated by apportioning intensity of C1 peak areas between conformational types on the basis of ordered and amorphous subspectra of the native starch. Quantitative analysis shows that the V-type single-helical component increases with amylose content of starches. Different amorphous subspectra are needed to provide a consistent analysis of granular starches from diverse sources. The method of preparation was found to be more important than the starch botanical origin in determining (13)C NMR spectral features of amorphous samples.
Ihwa Tan; Bernadine M Flanagan; Peter J Halley; Andrew K Whittaker; Michael J Gidley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-01
Journal Detail:
Title:  Biomacromolecules     Volume:  8     ISSN:  1525-7797     ISO Abbreviation:  Biomacromolecules     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-12     Completed Date:  2007-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  885-91     Citation Subset:  IM    
Centre for High Performance Polymers, The University of Queensland, St Lucia QLD 4072, Australia.
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MeSH Terms
Biochemistry / methods*
Carbon Isotopes / chemistry
Macromolecular Substances
Magnetic Resonance Spectroscopy / methods*
Models, Chemical
Molecular Conformation
Molecular Structure
Starch / chemistry
X-Ray Diffraction
Zea mays / metabolism
Reg. No./Substance:
0/Carbon Isotopes; 0/Macromolecular Substances; 9005-25-8/Starch

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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