| A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems. | |
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MedLine Citation:
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PMID: 10585739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipases A(1) and A(2) frequently coexist in biological systems. Generation of lysophosphatidylcholine (LPC) in such systems cannot be assigned to any of these types of enzymes unless the position of the fatty acid in the lysocompound can be unambiguously determined. We here present a simple method to achieve this purpose. It is based on the initial chemical acylation of the isolated LPC with a labeled fatty acid, followed by the enzymatic analysis of the resulting phosphatidylcholine (PC), using snake or bee venom phospholipase A(2). Thus, if treatment of the PC with this enzyme releases a labeled free fatty acid, it is demonstrated that the initial LPC was acylated at position sn-1, whereas if the product of hydrolysis yields labeled LPC, then the initial LPC was acylated at position sn-2. This is the first method devised to determine the source of LPC in the presence of mixtures of phospholipases A(1) and A(2) in complex biological systems. |
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Authors:
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J Florin-Christensen; J Narvaez-Vasquez; M Florin-Christensen; C A Ryan |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 276 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-14 Completed Date: 2000-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 13-7 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164-7040, USA. jfc@vetmed.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acylation Animals Cattle Enzyme Activation Erythrocyte Membrane / metabolism Hydrolysis Lysophosphatidylcholines / analysis*, biosynthesis, chemistry* Molecular Structure Phospholipases A / metabolism Tetrahymena thermophila / enzymology Trypanosoma cruzi / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lysophosphatidylcholines; EC 3.1.1.-/Phospholipases A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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