Document Detail

A method for distinguishing 1-acyl from 2-acyl lysophosphatidylcholines generated in biological systems.
MedLine Citation:
PMID:  10585739     Owner:  NLM     Status:  MEDLINE    
Phospholipases A(1) and A(2) frequently coexist in biological systems. Generation of lysophosphatidylcholine (LPC) in such systems cannot be assigned to any of these types of enzymes unless the position of the fatty acid in the lysocompound can be unambiguously determined. We here present a simple method to achieve this purpose. It is based on the initial chemical acylation of the isolated LPC with a labeled fatty acid, followed by the enzymatic analysis of the resulting phosphatidylcholine (PC), using snake or bee venom phospholipase A(2). Thus, if treatment of the PC with this enzyme releases a labeled free fatty acid, it is demonstrated that the initial LPC was acylated at position sn-1, whereas if the product of hydrolysis yields labeled LPC, then the initial LPC was acylated at position sn-2. This is the first method devised to determine the source of LPC in the presence of mixtures of phospholipases A(1) and A(2) in complex biological systems.
J Florin-Christensen; J Narvaez-Vasquez; M Florin-Christensen; C A Ryan
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  276     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-14     Completed Date:  2000-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13-7     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164-7040, USA.
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MeSH Terms
Enzyme Activation
Erythrocyte Membrane / metabolism
Lysophosphatidylcholines / analysis*,  biosynthesis,  chemistry*
Molecular Structure
Phospholipases A / metabolism
Tetrahymena thermophila / enzymology
Trypanosoma cruzi / metabolism
Reg. No./Substance:
0/Lysophosphatidylcholines; EC 3.1.1.-/Phospholipases A

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