Document Detail


A method for determining cell number in the undisturbed epithelium of the mouse lens.
MedLine Citation:
PMID:  21139698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The anterior face of the mouse lens is covered by a layer of epithelial cells. The epithelial cells serve a barrier function at the lens surface and as a progenitor population from which lens fiber cells, the predominant cell type of the lens, are derived. Decreased epithelial cell density is commonly observed during aging and cataract formation in humans and animal models and may contribute directly to tissue opacification. However, the loss of cells from the epithelium is often not easy to quantify, in part because the cells are arrayed across a near-spherical surface and, as a consequence, are difficult to image and count. Here, we describe a technique for determining epithelial cell number in the undisturbed lens of the mouse, a popular cataract model. The method utilizes orthographic projections of confocal images collected from the anterior and equatorial regions of the lens. The overlapping projections are brought into register using the unique distribution of proliferating cells as fiduciary points. Cell counts are performed using a computer-assisted method. This approach offers several advantages over flat-mount methods employed previously.
Authors:
Steven Bassnett; Yanrong Shi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-11-04
Journal Detail:
Title:  Molecular vision     Volume:  16     ISSN:  1090-0535     ISO Abbreviation:  Mol. Vis.     Publication Date:  2010  
Date Detail:
Created Date:  2010-12-08     Completed Date:  2011-02-08     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9605351     Medline TA:  Mol Vis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2294-300     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63117, USA. Bassnett@vision.wustl.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count / methods*
Cell Nucleus / metabolism
Epithelial Cells / cytology*
Lens, Crystalline / cytology*
Mice
Mice, Inbred C57BL
Grant Support
ID/Acronym/Agency:
EY009852/EY/NEI NIH HHS; P30EY002687,/EY/NEI NIH HHS; R01EY018185/EY/NEI NIH HHS
Comments/Corrections

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