| A method for determination of total glutathione and total cysteine as S-carboxymethyl derivatives by using an amino acid analyzer, and its application to samples from rat liver, kidney and blood after intraperitoneal administration of 2-(4-carboxy-D-gluco-tetrahydroxybutyl)thiazolidine-4-carboxylic acid. | |
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MedLine Citation:
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PMID: 7762408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of intraperitoneal administration of 2-(4-carboxy-D-gluco-tetrahydroxybutyl)thiazolidine-4-carboxylic acid (CGUA), a cysteine derivative conjugated with glucuronic acid, on total glutathione and total cysteine contents in rat tissues were investigated. Total glutathione (GSH and GSSG) and total cysteine (cysteine and cystine) were determined by a new method consisting of preparation of S-carboxymethylglutathione (CMSG) and S-carboxymethylcysteine (CMC), respectively, and subsequent analyses with an amino acid analyzer. CGUA was determined by a coloration method employing an acidic ninhydrin reagent. Total cysteine contents in liver, kidney and plasma rapidly increased to 2.3, 2.7 and 6.5 times the levels of the controls, respectively, after CGUA administration at a dose of 5 mmol/kg of body weight. Total glutathione content did not change significantly in the liver or blood except for the kidney with a significant increase during the first 1-h period after administration. CGUA content increased markedly in these tissues, especially in the kidney, and 30% of administered CGUA was excreted in urine within 2h. These results indicate that CGUA is converted into cysteine in vivo, suggesting the usefulness of this compound for protection of the kidney and the liver. |
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Authors:
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Y Q Zhao; M Kinuta; T Abe; W B Yao; T Ubuka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta medica Okayama Volume: 49 ISSN: 0386-300X ISO Abbreviation: Acta Med. Okayama Publication Date: 1995 Feb |
Date Detail:
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Created Date: 1995-06-28 Completed Date: 1995-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417611 Medline TA: Acta Med Okayama Country: JAPAN |
Other Details:
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Languages: eng Pagination: 35-42 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Okayama University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Cysteine / analysis*, blood Glutathione / analysis*, blood Injections, Intraperitoneal Kidney / cytology, drug effects, metabolism* Liver / cytology, drug effects, metabolism* Male Rats Rats, Wistar Spectrophotometry Thiazoles / metabolism, pharmacology* Thiazolidines |
| Chemical | |
Reg. No./Substance:
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0/Thiazoles; 0/Thiazolidines; 444-27-9/thiazolidine-4-carboxylic acid; 52-90-4/Cysteine; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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