Document Detail

An LC/MS method for d8-β-carotene and d4-retinyl esters: β-carotene absorption and its conversion to vitamin A in humans.
MedLine Citation:
PMID:  22308509     Owner:  NLM     Status:  MEDLINE    
The intestinal absorption and metabolism of β-carotene is of vital importance in humans, especially in populations that obtain the majority of their vitamin A from provitamin A carotenoids. MS has provided a better understanding of the absorption of β-carotene, the most potent provitamin A carotenoid, through the use of stable isotopes of β-carotene. We report here an HPLC-MS method that eliminates the need for complicated sample preparation and allows us to detect and quantify newly absorbed d8-β-carotene as well as its d4-retinyl ester metabolites in human plasma and chylomicron fractions. Both retinoids and β-carotene were recovered in a single simple extraction that did not involve saponification, thus allowing subsequent quantitation of individual fatty acyl esters of retinol. Separation of d8-β-carotene and its d4-retinyl ester metabolites was achieved using the same C30 reversed-phase liquid chromatography followed by mass spectrometry in selected ion monitoring and negative atmospheric pressure chemical ionization modes, respectively. Total time for the two successive runs was 30 min. This HPLC-MS method allowed us to quantify the absorption of intact d8-β-carotene as well as its extent of conversion to d4-retinyl esters in humans after consumption of a single 5 mg dose of d8-β-carotene.
Matthew K Fleshman; Ken M Riedl; Janet A Novotny; Steven J Schwartz; Earl H Harrison
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Publication Detail:
Type:  Journal Article     Date:  2012-02-03
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-13     Completed Date:  2012-07-02     Revised Date:  2013-09-05    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  820-7     Citation Subset:  IM    
Department of Human Nutrition, The Ohio State University, Columbus, OH, USA.
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MeSH Terms
Atmospheric Pressure
Carotenoids / metabolism
Cholesterol / metabolism
Chromatography, Reverse-Phase / methods*,  standards
Mass Spectrometry / methods*,  standards
Molecular Structure
Retinoids / blood,  chemistry*
Time Factors
Vitamin A / metabolism*
beta Carotene / administration & dosage,  blood,  metabolism*
Grant Support
Reg. No./Substance:
0/Retinoids; 11103-57-4/Vitamin A; 36-88-4/Carotenoids; 57-88-5/Cholesterol; 7235-40-7/beta Carotene; SB0N2N0WV6/lycopene

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