| A method for the continuous purification of proteins by affinity adsorption. | |
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MedLine Citation:
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PMID: 7764301 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method was conceived for the purification of biomolecules on a continuous-flow basis using affinity adsorption. The affinity ligand was bound to a nylon belt which was passed sequentially through four chambers to which flows of feedstock, wash medium, eluent and regeneration medium were applied. The target compound was thus removed from the feedstock stream and desorbed into a continuous flow of eluent. Prototype laboratory-scale apparatus was designed and constructed and the technical feasibility of this method was demonstrated using soybean trypsin inhibitor as a ligand for the adsorption of trypsin. The effects of various operational parameters on apparatus function were investigated using this model system. In addition, continuous removal of trypsin from a bovine pancreatic extract was carried out during an 8 h experiment. |
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Authors:
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G W Niven; P G Scurlock |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biotechnology Volume: 31 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1994-02-08 Completed Date: 1994-02-08 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 179-90 Citation Subset: B |
Affiliation:
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AFRC Institute of Food Research, Reading Laboratory, Earley Gate, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals Cattle Chemistry Techniques, Analytical / instrumentation, methods Pancreas / chemistry Proteins / isolation & purification* Trypsin / isolation & purification Trypsin Inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/Trypsin Inhibitors; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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