| A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran. | |
| | |
MedLine Citation:
|
PMID: 17953987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The assessment of blood volume parameters in clinical and research settings has been limited by methods that involve radioactivity, complex assays or are unreliable. We aimed to design a method for measuring blood volume parameters that was non-radioactive, simple, cheap and reliable. We have used a commercially available fluorescein-labelled 250kDa dextran, a large inert molecule, and have measured dilution of this through the intravascular space of pregnant ewes. From this estimation of plasma volume and measured hematocrit, we have calculated blood volume and red cell volume. The blood volume results are 6% lower than those obtained using radiolabelled red cells, but there is no significant difference in red cell volume between methods. The coefficient of variation for repeated measurements of plasma volume measurements is 3.8%. This is a simple, reliable, cheap and non-radioactive method for estimating blood volume parameters in pregnant sheep, and may prove useful in other settings. |
| | |
Authors:
|
C W H Rumball; P Van Zijl; M D Rutland; F H Bloomfield; J E Harding |
Related Documents
:
|
12679727 - Absolute cerebral blood volume and blood flow measurements based on synchrotron radiati... 2315797 - The effect of plasma volume expansion on uteroplacental blood flow in hypertensive preg... 3402187 - Regional blood volume in man determined by radiolabelled erythrocytes. 9746407 - Hematuria associated with low-volume cell saver in pediatric orthopaedics. 2008657 - Tissue oxygen tension and other indicators of blood loss or organ perfusion during grad... 7303077 - Cerebral oxygen availability and blood flow during middle cerebral artery occlusion: ef... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article Date: 2007-10-22 |
Journal Detail:
|
Title: Placenta Volume: 29 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2008 Jan |
Date Detail:
|
Created Date: 2008-01-14 Completed Date: 2008-03-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
|
Languages: eng Pagination: 15-9 Citation Subset: IM |
Affiliation:
|
The Liggins Institute, University of Auckland, Private Bag 92019, Auckland, New Zealand. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Volume Determination / methods* Dextrans / blood*, pharmacokinetics Female Fluorescein-5-isothiocyanate / analogs & derivatives*, pharmacokinetics Pregnancy Pregnancy, Animal / blood* Sheep, Domestic / blood* |
| Chemical | |
Reg. No./Substance:
|
0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenoty...
Next Document: Rapid assessment of avoidable blindness in Kunming, china.