Document Detail

The metalloprotease of Listeria monocytogenes is activated by intramolecular autocatalysis.
MedLine Citation:
PMID:  17965168     Owner:  NLM     Status:  MEDLINE    
The metalloprotease (Mpl) of Listeria monocytogenes is a thermolysin-like protease that mediates the maturation of a broad-range phospholipase C, whose function contributes to the ability of this food-borne bacterial pathogen to survive intracellularly. Mpl is made as a proprotein that undergoes maturation by proteolytic cleavage of a large N-terminal prodomain. In this study, we identified the N terminus of mature Mpl and generated Mpl catalytic mutants to investigate the mechanism of Mpl maturation. We observed that Mpl activity was a prerequisite for maturation, suggesting a mechanism of autocatalysis. Furthermore, using a strain of L. monocytogenes expressing both the wild-type form and a catalytic mutant form of Mpl simultaneously, we determined that in vivo maturation of Mpl occurs exclusively by an intramolecular autocatalysis mechanism.
Alan Pavinski Bitar; Min Cao; Hélène Marquis
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-10-26
Journal Detail:
Title:  Journal of bacteriology     Volume:  190     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-21     Completed Date:  2008-01-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  107-11     Citation Subset:  IM    
Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / metabolism
DNA Primers
Enzyme Activation
Listeria monocytogenes / enzymology*,  genetics
Metalloproteases / genetics,  metabolism*
Molecular Sequence Data
Mutagenesis, Site-Directed
Peptide Fragments / chemistry
Recombinant Proteins / metabolism
Type C Phospholipases / metabolism
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Primers; 0/Peptide Fragments; 0/Recombinant Proteins; EC 3.1.4.-/Type C Phospholipases; EC 3.4.-/Metalloproteases

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