| The metalloprotease of Listeria monocytogenes is activated by intramolecular autocatalysis. | |
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MedLine Citation:
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PMID: 17965168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metalloprotease (Mpl) of Listeria monocytogenes is a thermolysin-like protease that mediates the maturation of a broad-range phospholipase C, whose function contributes to the ability of this food-borne bacterial pathogen to survive intracellularly. Mpl is made as a proprotein that undergoes maturation by proteolytic cleavage of a large N-terminal prodomain. In this study, we identified the N terminus of mature Mpl and generated Mpl catalytic mutants to investigate the mechanism of Mpl maturation. We observed that Mpl activity was a prerequisite for maturation, suggesting a mechanism of autocatalysis. Furthermore, using a strain of L. monocytogenes expressing both the wild-type form and a catalytic mutant form of Mpl simultaneously, we determined that in vivo maturation of Mpl occurs exclusively by an intramolecular autocatalysis mechanism. |
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Authors:
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Alan Pavinski Bitar; Min Cao; Hélène Marquis |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-10-26 |
Journal Detail:
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Title: Journal of bacteriology Volume: 190 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-21 Completed Date: 2008-01-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 107-11 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Proteins / metabolism Catalysis DNA Primers Enzyme Activation Genotype Kinetics Listeria monocytogenes / enzymology*, genetics Metalloproteases / genetics, metabolism* Molecular Sequence Data Mutagenesis, Site-Directed Peptide Fragments / chemistry Recombinant Proteins / metabolism Type C Phospholipases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI52154/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA Primers; 0/Peptide Fragments; 0/Recombinant Proteins; EC 3.1.4.-/Type C Phospholipases; EC 3.4.-/Metalloproteases |
| Comments/Corrections | |
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