Document Detail

A metal dispersed sol-gel biocomposite amperometric glucose biosensor.
MedLine Citation:
PMID:  12706584     Owner:  NLM     Status:  MEDLINE    
A new glucose biosensor has been fabricated by immobilizing glucose oxidase into a copper dispersed sol-gel derived ceramic-graphite composite. The copper in the biocomposite offers excellent electrocatalytic activity towards the reduction (at -0.2 V) as well as oxidation (at +0.45 V) of hydrogen peroxide liberated in the enzymatic reaction enabling sensitive and selective determination of glucose. A linear response to glucose in the concentration range between 2.7 x 10(-5) to 4.0 x 10(-3) M with a correlation coefficient of 0.9987 and 4.0 x 10(-5) to 5.6 x 10(-3) M with a correlation coefficient of 0.9989 were observed with the electrocatalytic reduction and oxidation, respectively. Ascorbic acid and uric acid did not interfere with the glucose measurement during catalytic reduction at -0.2 V, a Nafion membrane was used to eliminate these interferences during the electrocatalytic oxidation at +0.45 V. The combination of copper catalysis and the promising feature of sol-gel biocomposite favor the sensitive and selective determination of glucose with improved analytical capabilities.
D Ravi Shankaran; N Ueheara; T Kato
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  18     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2004-04-13     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  721-8     Citation Subset:  IM    
Satellite Venture Business Laboratory, Utsunomiya University, Yoto 7-1-2, 321-8585, Utsunomiya, Japan.
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MeSH Terms
Biosensing Techniques / instrumentation*,  methods
Ceramics / chemistry
Coated Materials, Biocompatible / chemical synthesis*,  chemistry
Copper / chemistry*
Electrochemistry / instrumentation*,  methods
Enzymes, Immobilized / chemistry
Equipment Failure Analysis
Fluorocarbon Polymers / chemistry
Gels / chemistry
Glucose / analysis*,  chemistry
Glucose Oxidase / chemistry*
Hydrogen Peroxide / analysis,  chemistry
Phase Transition
Reproducibility of Results
Sensitivity and Specificity
Silanes / chemistry*
Reg. No./Substance:
0/Ceramics; 0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 0/Fluorocarbon Polymers; 0/Gels; 0/Silanes; 39464-59-0/perfluorosulfonic acid; 4420-74-0/(3-mercaptopropyl)trimethoxysilane; 50-99-7/Glucose; 7440-50-8/Copper; 7722-84-1/Hydrogen Peroxide; 7782-42-5/Graphite; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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