Document Detail


A metabolomic approach to dissecting osmotic stress in the wheat pathogen Stagonospora nodorum.
MedLine Citation:
PMID:  18796335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A non-targeted metabolomics approach was used to identify significant changes in metabolism upon exposure of the wheat pathogen Stagonospora nodorum to 0.5M NaCl. The polyol arabitol, and to a lesser extent glycerol, was found to accumulate in response to the osmotic stress treatment. Amino acid synthesis was strongly down-regulated whilst mannitol levels were unaffected. A reverse genetic approach was undertaken to dissect the role of arabitol metabolism during salt stress. Strains of S. nodorum lacking a gene encoding an l-arabitol dehydrogenase (abd1), a xylitol dehydrogenase (xdh1) and a double-mutant lacking both genes (abd1xdh1) were exposed to salt and the intracellular metabolites analysed. Arabitol levels were significantly up-regulated upon salt stress in the xdh1 strains but were significantly lower than the wild-type. Arabitol was not significantly different in either the abd1 or the abd1xdh1 strains during osmotic stress but the concentration of glycerol was significantly higher indicating a compensatory mechanism in operation. Genome sequence analysis identified a second possible enzyme capable of synthesizing arabitol explaining the basal level of arabitol present in the abd1xdh1 strains. This study identified that arabitol is the primary compatible solute in S. nodorum but in-built levels of redundancy are present allowing the fungus to tolerate osmotic stress.
Authors:
Rohan G T Lowe; Maryn Lord; Kasia Rybak; Robert D Trengove; Richard P Oliver; Peter S Solomon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-29
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  45     ISSN:  1096-0937     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-10     Completed Date:  2008-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1479-86     Citation Subset:  IM    
Affiliation:
Australian Centre for Necrotrophic Fungal Pathogens, SABC, Division of Health Sciences, Murdoch University, Perth, WA, 6150, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / biosynthesis
Ascomycota / enzymology,  genetics,  metabolism*
Fungal Proteins / genetics,  metabolism
Osmotic Pressure
Plant Diseases / microbiology*
Sodium Chloride / metabolism
Sugar Alcohol Dehydrogenases / genetics,  metabolism
Sugar Alcohols / metabolism
Triticum / microbiology*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fungal Proteins; 0/Sugar Alcohols; 2152-56-9/arabitol; 7647-14-5/Sodium Chloride; EC 1.1.-/Sugar Alcohol Dehydrogenases

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