| The metabolism and excretion of enzyme-inducing doses of phenobarbital by rats with bile fistulas. | |
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MedLine Citation:
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PMID: 3705618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of enzyme-inducing doses of 14C-phenobarbital injected i. p. into bile duct-cannulated rats has been studied using improved chromatographic separation and quantification techniques. In animals with bile fistulas most of the 14C-phenobarbital was excreted in bile as p-hydroxyphenobarbital conjugated with glucuronic acid. In urine the main substance found was phenobarbital, with significant amounts of p-hydroxyphenobarbital and varying amounts of its glucuronide conjugate. Animals without bile fistulas excreted 80% dose of phenobarbital in the urine; metabolites were free phenobarbital, p-hydroxyphenobarbital and conjugated material. Approx 90% of the conjugated material was the glucuronide. Only free phenobarbital and p-hydroxyphenobarbital were found in the faeces. Animals drinking plain water excreted 50-65% dose of phenobarbital (80 mg/kg) in bile and the remainder mainly in the urine, whereas superhydrated animals (drinking 5% glucose and 0.9% NaCl) excreted 90% of the dose as free phenobarbital in the urine. Phenobarbital is the only labelled material detectable in hepatic tissue and portal, vena caval or aortic blood, which indicates that phenobarbital is the enzyme-inducing substance and that liver and kidney rapidly eliminate all metabolites. Metabolism of phenobarbital in vivo is a complex process involving interaction of hepatic and intestinal metabolism, partial readsorption from the intestinal tract and renal elimination. |
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Authors:
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S S Levin; H M Vars; H Schleyer; D Y Cooper |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Xenobiotica; the fate of foreign compounds in biological systems Volume: 16 ISSN: 0049-8254 ISO Abbreviation: Xenobiotica Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-05-28 Completed Date: 1986-05-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1306665 Medline TA: Xenobiotica Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 213-24 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / metabolism* Biliary Fistula / metabolism Biotransformation Cytochrome P-450 Enzyme System / metabolism Glucuronates / metabolism Liver / metabolism Male Mixed Function Oxygenases / metabolism Phenobarbital / analogs & derivatives, metabolism*, urine Rats Subcellular Fractions / metabolism Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Glucuronates; 379-34-0/4-hydroxyphenobarbital; 50-06-6/Phenobarbital; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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