Document Detail

The metabolism of D- and L-3-hydroxybutyrate in developing rat brain.
MedLine Citation:
PMID:  6477538     Owner:  NLM     Status:  MEDLINE    
The incorporation of L- and D-3-hydroxybutyrate into rat brain protein, lipid, and amino acids during development was studied. L-3-Hydroxybutyrate was found to label brain protein and amino acids in addition to sterols and fatty acids throughout the first 32 postnatal days. Age related changes in L- and D-3-hydroxybutyrate labeling of protein and amino acids were similar. Whereas L-3-hydroxybutyrate incorporation into brain lipids rose sharply between 6-15 days of age, D-3-HOB incorporation into the lipid fraction gradually increased from birth through the age of 15 days. Incorporation by both isomers into lipid was greatest during the third week of suckling and then declined when the animals were weaned. At 15 days of age, the distribution of L-3-hydroxybutyrate into glutamate, glutamine + aspartate, and gamma-aminobutyrate was similar to that obtained with D-3-hydroxybutyrate. L-3-Hydroxybutyrate was poorly oxidized to CO2 by brain slices and mitochondria. Oxidation capacity was maximal from 15-21 days of age for both isomers. The activity of L-3-hydroxybutyrl-CoA ligase increased between 6-28 days of age, and its increase is well correlated with the developmental pattern of L-3-hydroxybutyrate incorporation and mitochondrial oxidation. L-3-Hydroxybutyrate was not detected in the blood of palmitate-injected pups or fasted adult animals. These results suggest that although L-3-hydroxybutyrate can be utilized for the synthesis of brain components during development, its negligible blood concentration precludes a significant contribution to either tissue synthesis or energy balance during the suckling period.
K R Swiatek; G J Dombrowski; K L Chao
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biochemical medicine     Volume:  31     ISSN:  0006-2944     ISO Abbreviation:  Biochem Med     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-10-10     Completed Date:  1984-10-10     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0151424     Medline TA:  Biochem Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  332-46     Citation Subset:  IM    
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MeSH Terms
3-Hydroxybutyric Acid
Brain / growth & development,  metabolism*
Coenzyme A Ligases / metabolism
Fatty Acids / biosynthesis
Hydroxybutyrates / metabolism*
Palmitates / metabolism
Rats, Inbred Strains
Sterols / biosynthesis
Reg. No./Substance:
0/Fatty Acids; 0/Hydroxybutyrates; 0/Palmitates; 0/Sterols; 300-85-6/3-Hydroxybutyric Acid; EC 6.2.1.-/Coenzyme A Ligases; EC synthetase

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