| The metabolism of 22:5(-6) and of docosahexaenoic acid [22:6(-3)] compared in rat hepatocytes. | |
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MedLine Citation:
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PMID: 8908151 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Elevated levels of 22:5(-6), which is the elongated and desaturated product of arachidonic acid, is induced by selective n-3 fatty acid deficiency, especially in brain cortex. Less elongation and desaturation of 20:4(-6) than of 20:5(-3) has been found in intact rat liver cells in previous studies and is probably the main reason why so little 22:5(-6) is found under adequate nutritional conditions. The present study compares the metabolism of 22:5(-6) with the metabolism of 22:6(-3), the main n-3 fatty acid in mammals. Freshly isolated rat liver cells were incubated with [1-14C]22:5(-6) and [1-14C]22:6(-3). Oxidation and esterification in triacylglycerols, diacylglycerols and phospholipids were studied. The phospholipid classes were separated and the different molecular species identified. Rats with essential fatty acid deficiency were compared with control rats. 22:5(-6) was found to be a good substrate for membrane phospholipid biosynthesis and was conserved well in the phospholipid fraction of the rat liver cells for more than 3 h of incubation. More 22:5(-6) was esterified in the total phospholipid fraction and less was incorporated in triacylglycerols than observed with 22:6(-3) in hepatocytes from control animals. This was not the case in animals with essential fatty acid deficiency. 22:5(-6) was esterified to a greater extent in phosphatidylcholine than 22:6(-3) in control cells but not in essential fatty acid deficiency cells. More 22:5(-6) was coupled with 18.0 in the sn-1 position of the phospholipid molecular species than 22:6(-3) was in control cells. |
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Authors:
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K Retterstøl; B Woldseth; B O Christophersen |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1303 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1996 Oct |
Date Detail:
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Created Date: 1996-12-06 Completed Date: 1996-12-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 180-6 Citation Subset: IM |
Affiliation:
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Institute of Clinical Biochemistry, University of Oslo, Rikshospitalet, Norway. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / metabolism* Diglycerides / metabolism Docosahexaenoic Acids / metabolism* Esterification Fatty Acids / metabolism Kinetics Liver / metabolism* Male Oxidation-Reduction Phospholipids / metabolism Rats Rats, Wistar Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Fatty Acids; 0/Phospholipids; 0/Triglycerides; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid |
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