Document Detail

A metabolic view of receptor activation in cultured cells following cryopreservation.
MedLine Citation:
PMID:  8403989     Owner:  NLM     Status:  MEDLINE    
The effect of cryopreservation on agonist-induced receptor activation in mammalian cells was investigated with the Cytosensor microphysiometer, a biosensor that monitors cellular metabolic activity by measuring changes in extracellular pH. In this study, two different cell types--nonadherent TF-1 cells (from a human erythroleukemia patient) and adherent WT3 cells (CHO-K1 cells transfected with the m1 muscarinic acetylcholine receptor)--were cryopreserved by freezing in a disposable cell capsule used in the microphysiometer. The recovery of metabolic activity by TF-1 cells was observed over approximately 1 h following thawing. Responses of the TF-1 cells to granulocyte-macrophage colony-stimulating factor (GM-CSF) and platelet activating factor (PAF) were measured before cryopreservation and 90 min after thawing. The GM-CSF and PAF responses retained 71 +/- 14% and 73 +/- 10% of maximum stimulation, respectively. Post-thaw cholinergic stimulation of WT3 cells was 73 +/- 9% of its level in similarly treated but unfrozen cells. Cryopreservation caused no detectable difference in desensitization of the response due to repeated application of carbachol. These results demonstrate the feasibility of pharmacological studies with cryopreserved cells in the microphysiometer and further suggest that the microphysiometer may be useful in exploring the biological consequences of cryopreservation in the early post-thaw period.
R C Kuo; G T Baxter; L Alajoki; D L Miller; J M Libby; J C Owicki
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  30     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-11-22     Completed Date:  1993-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  386-95     Citation Subset:  IM    
Molecular Devices Corp., Menlo Park, California 94025.
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MeSH Terms
CHO Cells / cytology,  drug effects,  metabolism
Carbachol / pharmacology
Cell Adhesion
Cell Survival
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
Platelet Activating Factor / pharmacology
Platelet Membrane Glycoproteins / drug effects,  metabolism*
Receptors, Cell Surface*
Receptors, G-Protein-Coupled*
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / drug effects,  metabolism*
Receptors, Muscarinic / drug effects,  metabolism*
Tumor Cells, Cultured / drug effects,  metabolism,  pathology
Reg. No./Substance:
0/Platelet Activating Factor; 0/Platelet Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; 0/Receptors, Muscarinic; 0/platelet activating factor receptor; 51-83-2/Carbachol; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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