Document Detail


A metabolic view of receptor activation in cultured cells following cryopreservation.
MedLine Citation:
PMID:  8403989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of cryopreservation on agonist-induced receptor activation in mammalian cells was investigated with the Cytosensor microphysiometer, a biosensor that monitors cellular metabolic activity by measuring changes in extracellular pH. In this study, two different cell types--nonadherent TF-1 cells (from a human erythroleukemia patient) and adherent WT3 cells (CHO-K1 cells transfected with the m1 muscarinic acetylcholine receptor)--were cryopreserved by freezing in a disposable cell capsule used in the microphysiometer. The recovery of metabolic activity by TF-1 cells was observed over approximately 1 h following thawing. Responses of the TF-1 cells to granulocyte-macrophage colony-stimulating factor (GM-CSF) and platelet activating factor (PAF) were measured before cryopreservation and 90 min after thawing. The GM-CSF and PAF responses retained 71 +/- 14% and 73 +/- 10% of maximum stimulation, respectively. Post-thaw cholinergic stimulation of WT3 cells was 73 +/- 9% of its level in similarly treated but unfrozen cells. Cryopreservation caused no detectable difference in desensitization of the response due to repeated application of carbachol. These results demonstrate the feasibility of pharmacological studies with cryopreserved cells in the microphysiometer and further suggest that the microphysiometer may be useful in exploring the biological consequences of cryopreservation in the early post-thaw period.
Authors:
R C Kuo; G T Baxter; L Alajoki; D L Miller; J M Libby; J C Owicki
Related Documents :
20047939 - Multi-scale heat and mass transfer modelling of cell and tissue cryopreservation.
16426599 - Relation of several antioxidant enzymes to rapid freezing resistance in suspension cult...
10529309 - Cryopreservation of keratinocytes in a monolayer.
8050269 - The influence of cryopreservation on parameters of energetic metabolism and motility of...
11061859 - Leukapheresis components may be cryopreserved at high cell concentrations without addit...
18601139 - Enhanced release of rosmarinic acid from coleus blumei permeabilized by dimethyl sulfox...
43269 - Isoelectric focusing of cells using zwitterionic buffers.
17004889 - Trans-10,cis-12 conjugated linoleic acid inhibits the g1-s cell cycle progression in du...
18682499 - Trpv4 enhances the cellular uptake of aminoglycoside antibiotics.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  30     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-11-22     Completed Date:  1993-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  386-95     Citation Subset:  IM    
Affiliation:
Molecular Devices Corp., Menlo Park, California 94025.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells / cytology,  drug effects,  metabolism
Carbachol / pharmacology
Cell Adhesion
Cell Survival
Cricetinae
Cryopreservation*
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
Humans
Kinetics
Platelet Activating Factor / pharmacology
Platelet Membrane Glycoproteins / drug effects,  metabolism*
Receptors, Cell Surface*
Receptors, G-Protein-Coupled*
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / drug effects,  metabolism*
Receptors, Muscarinic / drug effects,  metabolism*
Tumor Cells, Cultured / drug effects,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Platelet Activating Factor; 0/Platelet Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; 0/Receptors, Muscarinic; 0/platelet activating factor receptor; 51-83-2/Carbachol; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Morphological and functional alterations in endothelium, smooth muscle, and nerve fibers in rabbit a...
Next Document:  Corneal cryopreservation with chondroitin sulfate.