| The metabolic fate of red wine and grape juice polyphenols in humans assessed by metabolomics. | |
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MedLine Citation:
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PMID: 20013882 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolic impact of polyphenol-rich red wine and grape juice consumption in humans was studied using a metabolomics approach. Fifty-eight men and women participated in a placebo-controlled, double-crossover study in which they consumed during a period of 4 wk, either a polyphenol-rich 2:1 dry mix of red wine and red grape juice extracts (MIX) or only a grape juice extract (GJX). Twenty-four-hour urine samples were collected after each intervention. (1)H NMR spectroscopy was applied for global metabolite profiling, while GC-MS was used for focused profiling of urinary phenolic acids. Urine metabolic profiles after intake of both polyphenol-rich extracts were significantly differentiated from placebo using multilevel partial least squares discriminant analysis. A significant 35% increase in hippuric acid excretion (p<0.001) in urine was measured after the MIX consumption as) or only a red grape juice dry extract (GJX). 24-h urine samples were collected after each intervention. 1H-NMR spectroscopy was applied for global metabolite profiling, while gas chromatography-mass spectrometry (GC-MS) was used for focused profiling of urinary phenolic acids. Urine metabolic profiles after intake of both polyphenol-rich extracts were significantly differentiated from placebo using multilevel partial least squares discriminant analysis (ML-PLS-DA). A significant 35% increase in hippuric acid excretion (p<0.001) in urine was measured after the MIX consumption compared with placebo, whereas no change was found after GJX consumption. GC-MS-based metabolomics of urine allowed identification of 18 different phenolic acids, which were significantly elevated following intake of either extract. Syringic acid, 3- and 4-hydroxyhippuric acid and 4-hydroxymandelic acid were the strongest urinary markers for both extracts. MIX and GJX consumption had a slightly different effect on the excreted phenolic acid profile and on endogenous metabolite excretion, possibly reflecting their different polyphenol composition. |
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Authors:
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Ferdinand A van Dorsten; Christian H Grün; Ewoud J J van Velzen; Doris M Jacobs; Richard Draijer; John P M van Duynhoven |
Publication Detail:
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Type: Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: 897-908 Citation Subset: IM |
Affiliation:
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Unilever R&D Vlaardingen, NL-3130 AC Vlaardingen, The Netherlands. ferdi-van.dorsten@unilever.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Benzoic Acids / chemistry, urine Beverages / analysis* Biological Markers / chemistry, urine Biotransformation Cross-Over Studies Double-Blind Method Female Flavonoids / pharmacokinetics*, urine Fruit / chemistry* Gas Chromatography-Mass Spectrometry Humans Hypertension / prevention & control, urine Magnetic Resonance Spectroscopy Male Mandelic Acids / chemistry, urine Metabolomics / methods* Middle Aged Phenols / chemistry, pharmacokinetics*, urine Plant Extracts / administration & dosage, chemistry Vitis / chemistry* Wine / analysis* Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Benzoic Acids; 0/Biological Markers; 0/Flavonoids; 0/Mandelic Acids; 0/Phenols; 0/Plant Extracts; 0/polyphenols |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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