Document Detail


The metabolic fate of red wine and grape juice polyphenols in humans assessed by metabolomics.
MedLine Citation:
PMID:  20013882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The metabolic impact of polyphenol-rich red wine and grape juice consumption in humans was studied using a metabolomics approach. Fifty-eight men and women participated in a placebo-controlled, double-crossover study in which they consumed during a period of 4 wk, either a polyphenol-rich 2:1 dry mix of red wine and red grape juice extracts (MIX) or only a grape juice extract (GJX). Twenty-four-hour urine samples were collected after each intervention. (1)H NMR spectroscopy was applied for global metabolite profiling, while GC-MS was used for focused profiling of urinary phenolic acids. Urine metabolic profiles after intake of both polyphenol-rich extracts were significantly differentiated from placebo using multilevel partial least squares discriminant analysis. A significant 35% increase in hippuric acid excretion (p<0.001) in urine was measured after the MIX consumption as) or only a red grape juice dry extract (GJX). 24-h urine samples were collected after each intervention. 1H-NMR spectroscopy was applied for global metabolite profiling, while gas chromatography-mass spectrometry (GC-MS) was used for focused profiling of urinary phenolic acids. Urine metabolic profiles after intake of both polyphenol-rich extracts were significantly differentiated from placebo using multilevel partial least squares discriminant analysis (ML-PLS-DA). A significant 35% increase in hippuric acid excretion (p<0.001) in urine was measured after the MIX consumption compared with placebo, whereas no change was found after GJX consumption. GC-MS-based metabolomics of urine allowed identification of 18 different phenolic acids, which were significantly elevated following intake of either extract. Syringic acid, 3- and 4-hydroxyhippuric acid and 4-hydroxymandelic acid were the strongest urinary markers for both extracts. MIX and GJX consumption had a slightly different effect on the excreted phenolic acid profile and on endogenous metabolite excretion, possibly reflecting their different polyphenol composition.
Authors:
Ferdinand A van Dorsten; Christian H Grün; Ewoud J J van Velzen; Doris M Jacobs; Richard Draijer; John P M van Duynhoven
Publication Detail:
Type:  Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  897-908     Citation Subset:  IM    
Affiliation:
Unilever R&D Vlaardingen, NL-3130 AC Vlaardingen, The Netherlands. ferdi-van.dorsten@unilever.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Benzoic Acids / chemistry,  urine
Beverages / analysis*
Biological Markers / chemistry,  urine
Biotransformation
Cross-Over Studies
Double-Blind Method
Female
Flavonoids / pharmacokinetics*,  urine
Fruit / chemistry*
Gas Chromatography-Mass Spectrometry
Humans
Hypertension / prevention & control,  urine
Magnetic Resonance Spectroscopy
Male
Mandelic Acids / chemistry,  urine
Metabolomics / methods*
Middle Aged
Phenols / chemistry,  pharmacokinetics*,  urine
Plant Extracts / administration & dosage,  chemistry
Vitis / chemistry*
Wine / analysis*
Young Adult
Chemical
Reg. No./Substance:
0/Benzoic Acids; 0/Biological Markers; 0/Flavonoids; 0/Mandelic Acids; 0/Phenols; 0/Plant Extracts; 0/polyphenols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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