Document Detail


The mesothelial cell as a non-thrombogenic surface.
MedLine Citation:
PMID:  6523428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarly exposed to blood as a control were washed off the plastic.
Authors:
L J Nicholson; J M Clarke; R M Pittilo; S J Machin; N Woolf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  52     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-02-27     Completed Date:  1985-02-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  102-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Platelets
Blood Vessel Prosthesis* / adverse effects
Cells, Cultured
Endothelium / cytology*,  ultrastructure
Humans
Male
Microscopy, Electron, Scanning
Microvilli / ultrastructure
Omentum / cytology
Rats
Rats, Inbred Strains
Surface Properties
Thrombosis / etiology,  prevention & control*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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