| The mesothelial cell as a non-thrombogenic surface. | |
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MedLine Citation:
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PMID: 6523428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarly exposed to blood as a control were washed off the plastic. |
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Authors:
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L J Nicholson; J M Clarke; R M Pittilo; S J Machin; N Woolf |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Thrombosis and haemostasis Volume: 52 ISSN: 0340-6245 ISO Abbreviation: Thromb. Haemost. Publication Date: 1984 Oct |
Date Detail:
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Created Date: 1985-02-27 Completed Date: 1985-02-27 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7608063 Medline TA: Thromb Haemost Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 102-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Platelets Blood Vessel Prosthesis* / adverse effects Cells, Cultured Endothelium / cytology*, ultrastructure Humans Male Microscopy, Electron, Scanning Microvilli / ultrastructure Omentum / cytology Rats Rats, Inbred Strains Surface Properties Thrombosis / etiology, prevention & control* |
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