Document Detail


A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development.
MedLine Citation:
PMID:  19248165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of the cardiovascular system is a highly dynamic process dependent on multiple signaling pathways regulating proliferation, differentiation, migration, cell-cell and cell-matrix interactions. To characterize cell and tissue dynamics during the formation of the cardiovascular system in mice, we generated a novel transgenic mouse line, Tg(Flk1::myr-mCherry), in which endothelial cell membranes are brightly labeled with mCherry, a red fluorescent protein. Tg(Flk1::myr-mCherry) mice are viable, fertile, and do not exhibit any developmental abnormalities. High levels of mCherry are expressed in the embryonic endothelium and endocardium, and expression is also observed in capillaries in adult animals. Targeting of the fluorescent protein to the cell membrane allows for subcellular imaging and cell tracking. By acquiring confocal time lapses of live embryos cultured on the microscope stage, we demonstrate that the newly generated transgenic model beautifully highlights the sprouting behaviors of endothelial cells during vascular plexus formation. We have also used embryos from this line to imaging the endocardium in the beating embryonic mouse heart, showing that Tg(Flk1::myr-mCherry) mice are suitable for the characterization of cardio dynamics. Furthermore, when combined with the previously described Tg(Flk1::H2B-EYFP) line, cell number in addition to cell architecture is revealed, making it possible to determine how individual endothelial cells contribute to the structure of the vessel.
Authors:
Irina V Larina; Wei Shen; Olivia G Kelly; Anna-Katerina Hadjantonakis; Margaret H Baron; Mary E Dickinson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  292     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-05     Completed Date:  2009-05-19     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  333-41     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Vessels / embryology*,  metabolism
Cardiovascular System / embryology*,  metabolism
Cell Membrane / metabolism*
Cell Nucleus
Embryo, Mammalian / blood supply*,  metabolism
Endocardium
Endothelial Cells / metabolism*
Endothelium, Vascular
HeLa Cells
Humans
Luminescent Proteins / metabolism*
Mice
Mice, Transgenic
Vascular Endothelial Growth Factor Receptor-2 / physiology
Grant Support
ID/Acronym/Agency:
R01 DK084391-01/DK/NIDDK NIH HHS; R01 EB 02209/EB/NIBIB NIH HHS; R01 HD052115/HD/NICHD NIH HHS; R01 HD052115-01A1/HD/NICHD NIH HHS; R01 HD052115-05/HD/NICHD NIH HHS; R01 HL 077187/HL/NHLBI NIH HHS; R01 HL095586-01A1/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/red fluorescent protein; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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