Document Detail


A member of the p38 mitogen-activated protein kinase family is responsible for transcriptional induction of Dopa decarboxylase in the epidermis of Drosophila melanogaster during the innate immune response.
MedLine Citation:
PMID:  18519585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Drosophila innate immunity is controlled primarily by the activation of IMD (immune deficiency) or Toll signaling leading to the production of antimicrobial peptides (AMPs). IMD signaling also activates the JUN N-terminal kinase (JNK) cascade, which is responsible for immune induction of non-antimicrobial peptide immune gene transcription though the transcription factor AP-1. Transcription of the Dopa decarboxylase (Ddc) gene is induced in response to gram-negative and gram-positive septic injury, but not aseptic wounding. Transcription is induced throughout the epidermis and not specifically at the site of infection. Ddc transcripts are detectible within 2 h and remain high for several hours following infection with either gram-negative or gram-positive bacteria. Using Ddc-green fluorescent protein (GFP) reporter gene constructs, we show that a conserved consensus AP-1 binding site upstream of the Ddc transcription start site is required for induction. However, neither the Toll, IMD, nor JNK pathway is involved. Rather, Ddc transcription depends on a previously uncharacterized member of the p38 mitogen-activated protein kinase family, p38c. We propose that the involvement of DDC in a new pathway involved in Drosophila immunity increases the levels of dopamine, which is metabolized to produce reactive quinones that exert an antimicrobial effect on invading bacteria.
Authors:
Monica M Davis; David A Primrose; Ross B Hodgetts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-02
Journal Detail:
Title:  Molecular and cellular biology     Volume:  28     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-17     Completed Date:  2008-08-12     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4883-95     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Bacterial Infections / enzymology,  immunology,  microbiology
Binding Sites
Conserved Sequence
Dopa Decarboxylase / biosynthesis*,  chemistry,  genetics
Drosophila Proteins / metabolism
Drosophila melanogaster / enzymology*,  genetics,  immunology*,  microbiology
Enzyme Induction*
Epidermis / enzymology*,  immunology
Immunity, Innate / genetics
JNK Mitogen-Activated Protein Kinases / metabolism
MAP Kinase Signaling System
Molecular Sequence Data
Protein Binding
Survival Analysis
Time Factors
Toll-Like Receptors / metabolism
Transcription Factor AP-1 / metabolism
Transcription, Genetic*
p38 Mitogen-Activated Protein Kinases / metabolism*
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Toll-Like Receptors; 0/Transcription Factor AP-1; 0/immune deficiency protein, Drosophila; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 4.1.1.-/Dopa Decarboxylase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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