Document Detail

A member of the p38 mitogen-activated protein kinase family is responsible for transcriptional induction of Dopa decarboxylase in the epidermis of Drosophila melanogaster during the innate immune response.
MedLine Citation:
PMID:  18519585     Owner:  NLM     Status:  MEDLINE    
Drosophila innate immunity is controlled primarily by the activation of IMD (immune deficiency) or Toll signaling leading to the production of antimicrobial peptides (AMPs). IMD signaling also activates the JUN N-terminal kinase (JNK) cascade, which is responsible for immune induction of non-antimicrobial peptide immune gene transcription though the transcription factor AP-1. Transcription of the Dopa decarboxylase (Ddc) gene is induced in response to gram-negative and gram-positive septic injury, but not aseptic wounding. Transcription is induced throughout the epidermis and not specifically at the site of infection. Ddc transcripts are detectible within 2 h and remain high for several hours following infection with either gram-negative or gram-positive bacteria. Using Ddc-green fluorescent protein (GFP) reporter gene constructs, we show that a conserved consensus AP-1 binding site upstream of the Ddc transcription start site is required for induction. However, neither the Toll, IMD, nor JNK pathway is involved. Rather, Ddc transcription depends on a previously uncharacterized member of the p38 mitogen-activated protein kinase family, p38c. We propose that the involvement of DDC in a new pathway involved in Drosophila immunity increases the levels of dopamine, which is metabolized to produce reactive quinones that exert an antimicrobial effect on invading bacteria.
Monica M Davis; David A Primrose; Ross B Hodgetts
Related Documents :
10805795 - Lexa chimeras reveal the function of drosophila fos as a context-dependent transcriptio...
19028685 - Transcriptional synergy mediated by saf-1 and ap-1: critical role of n-terminal polyala...
23485525 - Toll-like receptors and myd88 adaptors in mytilus: complete cds and gene expression lev...
7750495 - Thyrotropin-releasing hormone and c-fos/c-jun genes are colocalized in rat anterior pit...
16786165 - Metallic but not ceramic wear particles increase prostaglandin e2 release and interleuk...
8382305 - Translation of equine infectious anemia virus bicistronic tat-rev mrna requires leaky r...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-02
Journal Detail:
Title:  Molecular and cellular biology     Volume:  28     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-17     Completed Date:  2008-08-12     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4883-95     Citation Subset:  IM    
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Bacterial Infections / enzymology,  immunology,  microbiology
Binding Sites
Conserved Sequence
Dopa Decarboxylase / biosynthesis*,  chemistry,  genetics
Drosophila Proteins / metabolism
Drosophila melanogaster / enzymology*,  genetics,  immunology*,  microbiology
Enzyme Induction*
Epidermis / enzymology*,  immunology
Immunity, Innate / genetics
JNK Mitogen-Activated Protein Kinases / metabolism
MAP Kinase Signaling System
Molecular Sequence Data
Protein Binding
Survival Analysis
Time Factors
Toll-Like Receptors / metabolism
Transcription Factor AP-1 / metabolism
Transcription, Genetic*
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/Drosophila Proteins; 0/Toll-Like Receptors; 0/Transcription Factor AP-1; 0/immune deficiency protein, Drosophila; EC Mitogen-Activated Protein Kinases; EC Mitogen-Activated Protein Kinases; EC 4.1.1.-/Dopa Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  MBD4-mediated glycosylase activity on a chromatin template is enhanced by acetylation.
Next Document:  Deletion of vascular endothelial growth factor C (VEGF-C) and VEGF-D is not equivalent to VEGF recep...