| A melanocyte-keratinocyte coculture model to assess regulators of pigmentation in vitro. | |
| | |
MedLine Citation:
|
PMID: 12054455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Many melanocyte or skin equivalent models have been used to evaluate the potential efficacy of melanogenic compounds to regulate pigmentation, but there has been great variation in results, partially stemming from the use of different cell lines and diverse conditions for the melanogenic assays. In an earlier report, we optimized a microtiter format assay system to screen potential bioactive compounds using immortalized melan-a melanocytes. That assay system, termed the STOPR protocol, allowed effects on melanocyte proliferation and differentiation to be assessed in a highly sensitive, reproducible, and cost-effective manner. However, in the skin and hair, melanocytes interact with keratinocytes, fibroblasts, and other cell types, and testing of putative bioactive compounds on melanocytes alone in culture does not allow one to observe the interactions with those other cell types, such as would occur in vivo. Therefore, we developed a melanocyte-keratinocyte coculture protocol that allows testing of compounds for potential effects on pigmentation in a more physiologically relevant context. It is a sensitive, reproducible, and reliable model for testing melanogenic regulators, and we have standardized it with known melanogenic inhibitors (hydroquinone, arbutin, kojic acid, and niacinamide) and stimulators (alpha-melanocyte-stimulating hormone, 8-methoxypsoralen, and 3,4-dihydroxyphenylalanine). This coculture system allows for large-scale screening of candidate compounds in conjunction with the STOPR protocol and provides a more physiologically relevant system to study melanocyte-keratinocyte interactions and to elucidate the regulatory mechanisms of melanogenic compounds. |
| | |
Authors:
|
Tie Chi Lei; Victoria M Virador; Wilfred D Vieira; Vincent J Hearing |
Related Documents
:
|
6440145 - Polycyclic aromatic hydrocarbon mutagenesis of human epidermal keratinocytes in culture. 1708735 - Three distinct keratinocyte subtypes identified in human oral epithelium by their patte... 1806035 - In vitro effects of beta-carotene on human oral keratinocytes from precancerous lesions... 9458905 - Smokeless tobacco-exposed oral keratinocytes increase macromolecular efflux from the in... 18430255 - Cell division in escherichia coli cultures monitored at single cell resolution. 17673545 - Retinoic acid signaling sensitizes hepatic stellate cells to nk cell killing via upregu... |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Analytical biochemistry Volume: 305 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2002 Jun |
Date Detail:
|
Created Date: 2002-06-10 Completed Date: 2002-12-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 260-8 Citation Subset: IM |
Copyright Information:
|
Copyright 2002 Elsevier Science (USA). |
Affiliation:
|
Pigment Cell Biology Section, Laboratory of Cell Biology, Bethesda, Maryland 20892, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arbutin / pharmacology Coculture Techniques / methods Dihydroxyphenylalanine / metabolism Hydroquinones / pharmacology Keratinocytes / drug effects, metabolism* Melanocyte-Stimulating Hormones / metabolism Melanocytes / drug effects, metabolism* Mice Models, Biological* Monophenol Monooxygenase / antagonists & inhibitors Niacinamide / pharmacology Pigmentation / physiology* Pyrones / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Hydroquinones; 0/Pyrones; 123-31-9/hydroquinone; 497-76-7/Arbutin; 501-30-4/kojic acid; 63-84-3/Dihydroxyphenylalanine; 9002-79-3/Melanocyte-Stimulating Hormones; 98-92-0/Niacinamide; EC 1.14.18.1/Monophenol Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chemical ionization mass spectrometric determination of acrolein in human breast cancer cells.
Next Document: High-performance liquid chromatographic technique for detection of a fluorescent analogue of ADP-rib...