Document Detail


The mediating role of cPLA2 in IL-1 beta and IL-6 release in LPS-induced HeLa cells.
MedLine Citation:
PMID:  14695653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies were conducted to characterize a HeLa cell model by which the roles of the 85-kDa phospholipase A2 (cPLA2) in interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) release could be evaluated. At first, untreated HeLa cells were compared with lipopolysaccharide (LPS)-treated HeLa cells. The latter resulted in cPLA2 overexpression and an increased trend of IL-1 beta and IL-6 release. The indicated doses of 85-kDa cPLA2 antisense oligonucleotide directed against the initiation site were then used to block cPLA2 in LPS-induced HeLa cells. The process led to a dose-dependent decrease in cPLA2 protein with no noticeable change of cPLA2 mRNA. Compared with that of LPS added only, a reduction of IL-1 beta and IL-6 levels in the supernatants of transfected cells following the repression of cPLA2 was observed. These results suggested that 85-kDa cPLA2 may mediate the signalling cascades by which IL-1 beta and IL-6 were released in LPS-induced HeLa cells.
Authors:
X H Wang; G T Yan; L H Wang; X H Hao; K Zhang; H Xue
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  22     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2003-12-25     Completed Date:  2004-08-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  41-4     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Affiliation:
Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, Beijing, PR China.
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MeSH Terms
Descriptor/Qualifier:
Hela Cells
Humans
Interleukin-1 / biosynthesis*
Interleukin-6 / biosynthesis*
Lipopolysaccharides / pharmacology*
Oligodeoxyribonucleotides, Antisense / pharmacology
Phospholipases A / antagonists & inhibitors,  metabolism*
Phospholipases A2
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
Radioimmunoassay
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Oligodeoxyribonucleotides, Antisense; 0/RNA, Messenger; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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