| A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. | |
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MedLine Citation:
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PMID: 11033548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the mechanism of inhibition of silver ions on microorganisms, two strains of bacteria, namely Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus), were treated with AgNO(3) and studied using combined electron microscopy and X-ray microanalysis. Similar morphological changes occurred in both E. coli and S. aureus cells after Ag(+) treatment. The cytoplasm membrane detached from the cell wall. A remarkable electron-light region appeared in the center of the cells, which contained condensed deoxyribonucleic acid (DNA) molecules. There are many small electron-dense granules either surrounding the cell wall or depositing inside the cells. The existence of elements of silver and sulfur in the electron-dense granules and cytoplasm detected by X-ray microanalysis suggested the antibacterial mechanism of silver: DNA lost its replication ability and the protein became inactivated after Ag(+) treatment. The slighter morphological changes of S. aureus compared with E. coli recommended a defense system of S. aureus against the inhibitory effects of Ag(+) ions. |
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Authors:
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Q L Feng; J Wu; G Q Chen; F Z Cui; T N Kim; J O Kim |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research Volume: 52 ISSN: 0021-9304 ISO Abbreviation: J. Biomed. Mater. Res. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2000-12-01 Completed Date: 2000-12-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0112726 Medline TA: J Biomed Mater Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 662-8 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Inc. |
Affiliation:
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Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology* Bacterial Proteins / analysis, drug effects* Cell Wall / drug effects*, ultrastructure Cytoplasmic Granules / ultrastructure DNA Replication / drug effects* DNA, Bacterial / analysis, drug effects* Electron Probe Microanalysis Escherichia coli / chemistry, drug effects*, ultrastructure Microscopy, Electron Silver / analysis Silver Nitrate / pharmacology* Staphylococcus aureus / chemistry, drug effects*, ultrastructure Sulfur / analysis |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/DNA, Bacterial; 7440-22-4/Silver; 7704-34-9/Sulfur; 7761-88-8/Silver Nitrate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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