Document Detail

The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae.
MedLine Citation:
PMID:  19826805     Owner:  NLM     Status:  MEDLINE    
A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.
Yong Chen; Shuya Li; Jian Xiong; Zhenjiang Li; Jianxin Bai; Lei Zhang; Qi Ye; Pingkai Ouyang; Hanjie Ying
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-14
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  86     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-16     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  75-81     Citation Subset:  IM    
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
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MeSH Terms
Biotechnology / methods*
Carbon / metabolism
Citric Acid / metabolism*,  pharmacology
Citric Acid Cycle / drug effects
Culture Media
Gene Expression Regulation, Enzymologic*
Glucose / metabolism*
Glycolysis / drug effects
Hydrogen-Ion Concentration
Orotic Acid / chemistry,  metabolism
Pentose Phosphate Pathway / drug effects
Saccharomyces cerevisiae / cytology,  growth & development,  metabolism*
Saccharomyces cerevisiae Proteins / biosynthesis,  metabolism
Uridine Monophosphate / biosynthesis*,  metabolism
Reg. No./Substance:
0/Culture Media; 0/Saccharomyces cerevisiae Proteins; 50-99-7/Glucose; 58-97-9/Uridine Monophosphate; 65-86-1/Orotic Acid; 7440-44-0/Carbon; 77-92-9/Citric Acid

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