| The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 19826805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle. |
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Authors:
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Yong Chen; Shuya Li; Jian Xiong; Zhenjiang Li; Jianxin Bai; Lei Zhang; Qi Ye; Pingkai Ouyang; Hanjie Ying |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-14 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 86 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-16 Completed Date: 2010-04-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 75-81 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods* Carbon / metabolism Catalysis Citric Acid / metabolism*, pharmacology Citric Acid Cycle / drug effects Culture Media Gene Expression Regulation, Enzymologic* Glucose / metabolism* Glycolysis / drug effects Hydrogen-Ion Concentration Orotic Acid / chemistry, metabolism Pentose Phosphate Pathway / drug effects Saccharomyces cerevisiae / cytology, growth & development, metabolism* Saccharomyces cerevisiae Proteins / biosynthesis, metabolism Uridine Monophosphate / biosynthesis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Saccharomyces cerevisiae Proteins; 50-99-7/Glucose; 58-97-9/Uridine Monophosphate; 65-86-1/Orotic Acid; 7440-44-0/Carbon; 77-92-9/Citric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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