Document Detail

A mechanism of the thearubigin fraction of black tea (Camellia sinensis) extract protecting against the effect of tetanus toxin.
MedLine Citation:
PMID:  12533914     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to elucidate the mechanism of the protective effect of black tea extract's thearubigin fraction against the action of tetanus toxin. The effects of thearubigin fraction extracted from a black tea infusion were examined for neuromuscular blocking action on tetanus toxin in mouse phrenic nerve-diaphragm preparations and on the binding of this toxin to the synaptosomal membrane preparations of rat cerebral cortices. The interaction between tetanus toxin and thearubigin fraction was also investigated. Tetanus toxin (4 micrograms/ml) abolished indirect twitches in mouse phrenic nerve-diaphragm preparations within 150 min. Thearubigin fraction mixed with tetanus toxin blocked the inhibitory effect of the toxin. Mixing iodinated toxin with thearubigin fraction inhibited the specific binding of [125I]tetanus toxin to the synaptosomal membrane preparation. The effects of thearubigin fraction were dose-dependent. The elution profile of [125I]tetanus toxin on Sephadex G-50 column chromatography was different from that of toxin mixed with thearubigin fraction. These findings indicate that thearubigin fraction protects against the action of tetanus toxin by binding with the toxin.
Eiki Satoh; Toshiaki Ishii; Yoshio Shimizu; Shin-ichi Sawamura; Masakazu Nishimura
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of toxicological sciences     Volume:  27     ISSN:  0388-1350     ISO Abbreviation:  J Toxicol Sci     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-01-21     Completed Date:  2003-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7805798     Medline TA:  J Toxicol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  441-7     Citation Subset:  IM    
Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine, 11, West-2, Inada, Obihiro 080-8555, Japan.
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MeSH Terms
Antioxidants / pharmacology*
Catechin / analogs & derivatives*,  pharmacology*
Diaphragm / drug effects
Neuromuscular Blocking Agents / pharmacology
Paralysis / chemically induced,  prevention & control
Phenols / pharmacology*
Phrenic Nerve / drug effects
Synaptosomes / drug effects
Tea / chemistry*
Tetanus Toxin / antagonists & inhibitors*,  toxicity*
Reg. No./Substance:
0/Antioxidants; 0/Neuromuscular Blocking Agents; 0/Phenols; 0/Tea; 0/Tetanus Toxin; 12698-96-3/thearubigin; 154-23-4/Catechin

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