Document Detail


The mechanism of hydralazine-induced collagen biosynthesis in cultured fibroblasts.
MedLine Citation:
PMID:  23344524     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The finding that hydralazine (HYD) affects collagen metabolism led us to investigate the mechanism of its action on collagen biosynthesis, prolidase expression and activity, expression of α(2)β(1) integrin, insulin-like growth factor-I receptor (IGF-IR), focal adhesion kinase (FAK), mitogen-activated protein (MAP) kinases (ERK(1), ERK(2)), and transcription factors hypoxia-inducible factor-1α (HIF-1α) and nuclear factor-κB p65 (NF-κB p65) in human dermal fibroblasts. Confluent fibroblasts were treated with micromolar concentrations (50-500 μM) of HYD for 24 h. HYD had no influence on cell viability. It was found that HYD-dependent increase in collagen biosynthesis was accompanied by a parallel increase in prolidase activity and expression, HIF-1α expression, and decrease in DNA biosynthesis, compared to untreated cells. Since collagen biosynthesis and prolidase activity are regulated by a signal induced by activated α(2)β(1) integrin receptor as well as IGF-IR, the expression of these receptors was measured by Western immunoblot analysis. The exposure of the cells to HYD contributed to the increase in IGF-IR expression without any effect on α(2)β(1) integrin receptor and FAK expressions. It was accompanied by a decrease in expression of MAP kinases and NF-κB p65, the known inhibitor of collagen gene expression. The data suggest that the HYD-dependent increase of collagen biosynthesis in cultured human skin fibroblasts results from activation of IGF-IR expression and prolidase activity and downregulation of NF-κB p65.
Authors:
Ewa Karna; Lukasz Szoka; Jerzy A Palka
Related Documents :
18339304 - Inhibition of osteocyte apoptosis by fluid flow is mediated by nitric oxide.
22533734 - Ribosomal protein l13a as a reference gene for human bone marrow-derived mesenchymal st...
22449704 - Glutaredoxin2 isoform b (glrx2b) promotes rankl-induced osteoclastogenesis through acti...
17973254 - Opposing effects of glucocorticoids and wnt signaling on krox20 and mineral deposition ...
18058464 - Inhibition of telomerase activity in cancer cells using short hairpin rna expression ve...
23865714 - Abnormal expression of pygopus 2 correlates with a malignant phenotype in human lung ca...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Naunyn-Schmiedeberg's archives of pharmacology     Volume:  -     ISSN:  1432-1912     ISO Abbreviation:  Naunyn Schmiedebergs Arch. Pharmacol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0326264     Medline TA:  Naunyn Schmiedebergs Arch Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Medicinal Chemistry, Medical University of Bialystok, Mickiewicza 2 D, 15-222, Bialystok, Poland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Outcome of Various Treatments for Posttransplant Hepatitis B Virus Recurrence.
Next Document:  TGF-?(1) T869C Polymorphism May Affect Susceptibility to Idiopathic Pulmonary Fibrosis and Disease S...