Document Detail


The mechanism of enhanced intraphagocytic killing of bacteria by liposomes containing antibiotics.
MedLine Citation:
PMID:  2336788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liposomes containing aminoglycosides have been shown to enhance the killing of Brucella abortus and Staphylococcus aureus inside bovine phagocytic cells. This study examined the mechanism by which liposomes containing aminoglycoside enhance the intracellular killing of bacteria. Liposomes with entrapped aminoglycoside were found to significantly enhance the intraphagocytic killing of bacteria in bovine phagocytic cells (in vitro) when compared to free drug. Liposomes with entrapped aminoglycoside were also found to deliver significantly higher levels of aminoglycoside into phagocytic cells when compared to free drug (gentamicin) or free drug and liposomes without entrapped antibiotic. Antibiotic delivered to adherent phagocytic cells could be detected 3 days after treatment of the cells with liposomes containing aminoglycoside. No antibiotic could be detected in the supernatants of phagocytic cell cultures 3 days after treatment with liposomes containing antibiotic was only observed when the intraphagocytic bacteria were sensitive to the antibiotic entrapped in the liposomes. The rate of phagocytosis of S. aureus by cells treated with cationic liposomes (no entrapped antibiotic) did not differ from the rate of phagocytosis of control cells not treated with cationic liposomes. This study shows that the enhanced intraphagocytic killing of bacteria in bovine phagocytic cells occurs by direct delivery of entrapped antibiotic into the phagocytic cell by the liposome delivery vehicle and not by nonspecific enhancement of phagocytic cell function. Liposomes containing aminoglycoside appear to have no toxic effects on phagocytic cell function or viability in vitro.
Authors:
C Dees; R D Schultz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  24     ISSN:  0165-2427     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-06-12     Completed Date:  1990-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  135-46     Citation Subset:  IM    
Affiliation:
Department of Microbiology, School of Veterinary Medicine, Auburn University, AL 36849.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cattle
Drug Carriers
Gentamicins / administration & dosage,  pharmacology*,  toxicity
Leukocytes, Mononuclear / metabolism
Liposomes / toxicity
Phagocytes / drug effects*
Staphylococcus aureus / drug effects*
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Gentamicins; 0/Liposomes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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