Document Detail


On the mechanism of differential Giemsa staining of BrdU-substituted chromatids.
MedLine Citation:
PMID:  6184205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper analyses the effect of acid hydrolysis on the differential Giemsa staining of 5-bromo-2'deoxyuridine (BrdU) substituted chromatids in human and plant chromosomes, after treatment with a fluorochrome and light. Human lymphocytes and Allium cepa L. root tips were grown in BrdU for two or three cell cycles. Lymphocytes spreadings and meristem squashes were treated with fluorochrome Hoechst 33258, exposed to sunlight, hydrolysed with 5N HCl and stained with giemsa. This acid hydrolysis improves the differential staining of BrdU substituted and non-substituted chromatin. It also allows the differentiation of sister chromatids with the DNA specific dye azure-A.
Authors:
G González-Gil; M H Navarrete
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosoma     Volume:  86     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1982  
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  375-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Azure Stains
Bromodeoxyuridine / pharmacology
Chromatids / drug effects,  ultrastructure*
DNA / metabolism
Fluorescent Dyes / pharmacology
Humans
Hydrochloric Acid / pharmacology
Lymphocytes / ultrastructure
Staining and Labeling / methods
Chemical
Reg. No./Substance:
0/Azure Stains; 0/Fluorescent Dyes; 59-14-3/Bromodeoxyuridine; 7647-01-0/Hydrochloric Acid; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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