| The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura. | |
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MedLine Citation:
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PMID: 18187043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera. METHODS: Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10% SDS-PAGE. The % area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS. RESULTS: Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p<0.01) was significantly higher in ITP patients than in non-ITP patients. We identified alpha(2)-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of alpha(2)-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p=0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively. CONCLUSIONS: Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state. |
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Authors:
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Mitsuhiro Chousa; Hiroyasu Ito; Kuniaki Saito; Kanako Takahashi; Masao Takemura; Takeshi Takahashi; Eiichi Tomita; Mitsuru Seishima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-14 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 389 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-01 Completed Date: 2008-05-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 132-8 Citation Subset: IM |
Affiliation:
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Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu City, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Blotting, Western Ceruloplasmin / analysis* Diagnosis, Differential Electrophoresis, Polyacrylamide Gel Female Humans Male Middle Aged Purpura, Thrombocytopenic, Idiopathic / blood, diagnosis* ROC Curve |
| Chemical | |
Reg. No./Substance:
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EC 1.16.3.1/Ceruloplasmin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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