Document Detail


The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura.
MedLine Citation:
PMID:  18187043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera. METHODS: Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10% SDS-PAGE. The % area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS. RESULTS: Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p<0.01) was significantly higher in ITP patients than in non-ITP patients. We identified alpha(2)-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of alpha(2)-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p=0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively. CONCLUSIONS: Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state.
Authors:
Mitsuhiro Chousa; Hiroyasu Ito; Kuniaki Saito; Kanako Takahashi; Masao Takemura; Takeshi Takahashi; Eiichi Tomita; Mitsuru Seishima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-14
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  389     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-01     Completed Date:  2008-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  132-8     Citation Subset:  IM    
Affiliation:
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu City, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blotting, Western
Ceruloplasmin / analysis*
Diagnosis, Differential
Electrophoresis, Polyacrylamide Gel
Female
Humans
Male
Middle Aged
Purpura, Thrombocytopenic, Idiopathic / blood,  diagnosis*
ROC Curve
Chemical
Reg. No./Substance:
EC 1.16.3.1/Ceruloplasmin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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