Document Detail

The measurement of the cell cycle time in squamous epithelium using the metaphase arrest technique with vincristine.
MedLine Citation:
PMID:  871384     Owner:  NLM     Status:  MEDLINE    
In squamous epithelia with a single layer of germinative cells, the age distribution of cells in the cell cycle is shown to depend on the direction of the mitotic axis (i.e. a line joining the nuclei of daughter cells) relative to the plane of the basal layer. When axes are in the plane of the basal layer the age distribution is exponential; when cells divide at right angles to the plane of the basal layer, the age distribution is rectangular. When there is a ratio of vertical to horizontal axes, the age distribution is intermediate but can be calculated from knowledge of the proportion of axes in the plane of the layer. Squamous epithelia can be classified according to this arrangement of axes. When there are multiple layers of germinative cells, as in psoriasis, the age distribution is shown to be exponential to a good approximation, whatever the direction of the mitotic axes in the several layers. The importance of these observations is demonstrated by analysing metaphase arrest experiments with vincristine in the single layer of germinative cells in the mouse oesophagus, and in the several layers found in psoriatic epidermis. Choice of the wrong age distribution leads to an error of 6 h in the oesophagus and 23 h in psoriatic epidermis, when the mean cell cycle time is calculated. It is concluded that, in squamous epithelium, it is most important to know the age distribution before calculating the cell cycle time by methods involving measurement of the rate of entry of cells into mitosis or DNA synthesis.
M B Duffill; D R Appleton; P Dyson; S Shuster; N A Wright
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of dermatology     Volume:  96     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-08-12     Completed Date:  1977-08-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  493-502     Citation Subset:  IM    
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MeSH Terms
Cell Division* / drug effects
Epithelial Cells*
Epithelium / physiology*
Esophagus / pathology
Mitosis / drug effects
Psoriasis / pathology
Time Factors
Vincristine / pharmacology
Reg. No./Substance:

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