Document Detail


A mathematical model for biopanning (affinity selection) using peptide libraries on filamentous phage.
MedLine Citation:
PMID:  8551746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mathematical model is presented for the process of selection of peptides for binding to a target. The affinity enrichment process commonly known as biopanning relies on subjecting a library of peptides on filamentous phage to a selection for binding to the target immobilized on solid support or in solution. The model is an implementation of the mass-action law to the diverse population of macromolecular assemblies. An analytical solution is presented for the enrichment process. Most parameters in the enrichment formula can be easily determined experimentally. Two examples corresponding to biopanning with epitope libraries and antibody libraries are given. The model allows for an estimation of the contribution of equilibrium and dissociative biopanning to the overall enrichment. The model can be a tool in the evaluation of the role of different biopanning parameters. Its implementation in a spreadsheet makes it possible to perform a computer simulation of a biopanning experiment.
Authors:
W Mandecki; Y C Chen; N Grihalde
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of theoretical biology     Volume:  176     ISSN:  0022-5193     ISO Abbreviation:  J. Theor. Biol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-02-20     Completed Date:  1996-02-20     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0376342     Medline TA:  J Theor Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  523-30     Citation Subset:  IM    
Affiliation:
Abbott Laboratories, North Chicago, IL 60064-4000, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / metabolism
Bacteriophages
Epitopes / metabolism
Models, Biological*
Models, Theoretical
Peptides / metabolism*
Protein Binding*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Epitopes; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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