Document Detail

A mass spectrometric study for comparative analysis and evaluation of metabolite recovery from plasma by various solvent systems.
MedLine Citation:
PMID:  23204928     Owner:  NLM     Status:  MEDLINE    
A solvent system that extracts a maximum number of metabolites belonging to diverse chemical classes from complex biofluids, such as plasma, may offer useful inputs to understand the metabolic and physiological state of an individual. The present study compared seven solvent systems for extraction of metabolites from plasma. The extracts were analyzed by mass spectrometry (MS) and MS/MS (MS2) using a quadrupole time-of-flight liquid chromatography/MS system in positive and negative modes of ionization. Metabolites with molecular mass below 400 were identified using Human Metabolome Database MS2 and MS search interfaces. The acetone/isopropanol (2:1) system yielded promising results in positive ionization mode, as the maximum number of MS and MS2 features was detected in the extract. It was found to be superior in extraction of various classes of metabolites, especially organic acids, nucleosides and nucleoside derivatives, and heterocyclic molecules. Glycerophosphocholines in the mass range of 400-700 were found to be efficiently extracted by the methanol/chloroform/water (8:1:1) system. In negative mode as well, the maximum number of MS2 features was detected in methanol/chloroform/water and acetone/isopropanol extracts. The fingerprints of molecular features obtained in the negative and positive modes differed from each other to a significant extent.
Anwesha Dutta; Premalatha Shetty; Smitha Bhat; Yeshaswini Ramachandra; Shrinidhi Hegde
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomolecular techniques : JBT     Volume:  23     ISSN:  1943-4731     ISO Abbreviation:  J Biomol Tech     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-03     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  100888641     Medline TA:  J Biomol Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  128-35     Citation Subset:  IM    
Department of Biotechnology, Manipal Life Sciences Centre, Manipal, India.
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MeSH Terms
2-Propanol / chemistry
Acetone / chemistry
Acetonitriles / chemistry
Blood Chemical Analysis / methods*
Chloroform / chemistry
Liquid-Liquid Extraction / methods*
Methanol / chemistry
Phosphatidylcholines / blood
Solvents / chemistry*
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry / methods*
Water / chemistry
Reg. No./Substance:
0/Acetonitriles; 0/Phosphatidylcholines; 0/Solvents; 67-56-1/Methanol; 67-63-0/2-Propanol; 67-64-1/Acetone; 67-66-3/Chloroform; 7732-18-5/Water; Z072SB282N/acetonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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