| A marked upregulation of uncoupling protein 2 gene expression in adipose tissue of hyperthyroid subjects. | |
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MedLine Citation:
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PMID: 11246812 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, a family of uncoupling protein (UCP) genes has been discovered. The role of these genes is unknown, but it has been suggested that they are involved in regulating resting metabolic rate. In this study, we hypothesised that thyroid hormone status may influence the expression of UCP2 mRNA. The adipose tissue levels of UCP2 mRNA were measured in eight female subjects before and after treatment for thyrotoxicosis. All subjects in the hyperthyroid condition had markedly enhanced plasma levels of thyroxine (62.0 +/- 6.9 vs. 17.9 +/- 1.7, p = 0.012) and triiodothyronine (37.9 +/- 6.9 vs. 5.9 +/- 0.9, p = 0.012), accelerated heart rate (94 +/- 7 vs. 69 +/- 5, p = 0.012), decreased BMI (24.5 +/- 1.9 vs. 25.1 +/- 1.9, p = 0.025) and decreased percentage body fat (32.8 +/- 4.4 vs. 37.1 +/- 4.5, p = 0.018), as compared to the euthyroid state. Using RT-competitive-PCR, the UCP2 mRNA levels were found to be 2.5-fold upregulated in hyperthyroidism (10.4 +/- 1.7 vs. 4.2 +/- 1.3 amol/microg RNA, p = 0.012). In contrast, no difference in expression levels of the reference gene 18SrRNA was seen in the hyperthyroid versus the euthyroid state (317 +/- 49 vs. 279 +/- 25 amol/microg RNA, p = 0.48) but the difference in UCP2 mRNA levels between the hyper- and euthyroid state remained when UCP2 was related to 18SrRNA (p = 0.012). In conclusion, thyrotoxicosis markedly increases the expression of UCP2 mRNA in adipose tissue, which suggests a role for thyroid hormones in the regulation of this uncoupling protein in man. |
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Authors:
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J Hoffstedt; R Folkesson; H Wahrenberg; A Wennlund; V van Harmelen; P Arner |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme Volume: 32 ISSN: 0018-5043 ISO Abbreviation: Horm. Metab. Res. Publication Date: 2000 Nov-Dec |
Date Detail:
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Created Date: 2001-03-14 Completed Date: 2001-05-31 Revised Date: 2009-02-19 |
Medline Journal Info:
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Nlm Unique ID: 0177722 Medline TA: Horm Metab Res Country: Germany |
Other Details:
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Languages: eng Pagination: 475-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, Karolinska Institutet, Huddinge University Hospital, Sweden. johan.hoffstedt@medhs.ki.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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chemistry,
metabolism* Adult Antithyroid Agents / therapeutic use Body Composition Body Mass Index Female Gene Expression Regulation* Heart Rate Humans Hyperthyroidism / drug therapy, physiopathology* Ion Channels Membrane Transport Proteins* Middle Aged Mitochondrial Proteins* Proteins / genetics* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Thyroxine / blood Triiodothyronine / blood Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Antithyroid Agents; 0/Ion Channels; 0/Membrane Transport Proteins; 0/Mitochondrial Proteins; 0/Proteins; 0/RNA, Messenger; 0/mitochondrial uncoupling protein 2; 6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine |
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