Document Detail

A mammary repopulating cell population characterized in mammary anlagen reveals essential mammary stroma for morphogenesis.
MedLine Citation:
PMID:  24954407     Owner:  NLM     Status:  Publisher    
The cells with mammary repopulating capability can achieve mammary gland morphogenesis in suitable cellular microenvironment. Using cell surface markers of CD24, CD29 and CD49f, mouse mammary repopulating unit (MRU) has been identified in adult mammary epithelium and late embryonic mammary bud epithelium. However, embryonic MRU remains to be fully characterized at earlier mammary anlagen stage. Here we isolated discrete populations of E14.5 mouse mammary anlagen cells. Only Lin(-)CD24(med)CD29(+) cell population was predicted as E14.5 MRU by examining their capacities of forming mammosphere and repopulating cleared mammary fat pad in vivo. However, when we characterized gene expressions of this E14.5 cell population by comparing with adult mouse MRU (Lin(-)CD24(+)CD29(hi)), the gene profiling of these two cell populations exhibited great differences. Real-time PCR and immunostaining assays uncovered that E14.5 Lin(-)CD24(med)CD29(+) cell population was a heterogeneous stroma-enriched cell population. Then, limiting dilutions and single-cell assays also confirmed that E14.5 Lin(-)CD24(med)CD29(+) cell population possessed low proportion of stem cells. In summary, heterogeneous Lin(-)CD24(med)CD29(+) cell population exhibited mammary repopulating ability in E14.5 mammary anlagen, implying that only suitable mammary stroma could enable mammary gland morphogenesis, which relied on the interaction between rare stem cells and microenvironment.
Jiazhe Song; Kai Xue; Ji She; Fangrong Ding; Song Li; Rulan Shangguan; Yunping Dai; Liying Du; Ning Li
Related Documents :
24349167 - Carnosine's effect on amyloid fibril formation and induced cytotoxicity of lysozyme.
22366957 - Recent progress in histochemistry and cell biology.
24849227 - Conversion of one cell type into another: implications for understanding organ developm...
23949017 - Δnp63 expression in four carcinoma cell lines and the effect on radioresistance-a sirna...
25232407 - Effect of stable transfection with phd3 on growth and proliferation of hepg2 cells in v...
2850247 - Vasopressin induces breakdown of phosphoinositides in adrenal tumor y-1 cells without a...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-19
Journal Detail:
Title:  Experimental cell research     Volume:  -     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The lid closure mechanism of Yarrowia Lipolytica Lipase in Methanol investigated by Molecular Dynami...
Next Document:  Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.