Document Detail

A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex.
MedLine Citation:
PMID:  14576168     Owner:  NLM     Status:  MEDLINE    
The multiprotein Mediator complex is a coactivator required for transcriptional activation of RNA polymerase II transcribed genes by DNA binding transcription factors. We previously partially purified a Med8-containing Mediator complex from rat liver nuclei (Brower, C. S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R. D., Malik, S., Lane, W. S., Sorokina, I., Roeder, R. G., Conaway, J. W., and Conaway, R. C. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10353-10358). Analysis of proteins present in the most highly enriched Mediator fractions by tandem mass spectrometry led to the identification of several new mammalian Mediator subunits, as well as several potential Mediator subunits. Here we identify one of these proteins, encoded by the previously uncharacterized AK000411 open reading frame, as a new subunit of the mammalian Mediator complex. The AK000411 protein, which we designate hIntersex (human Intersex), shares significant sequence similarity with the Drosophila melanogaster intersex protein, which has functional properties expected of a transcriptional coactivator specific for the Drosophila doublesex transactivator. In addition, we show that hIntersex assembles into a subcomplex with Mediator subunits p28b and TRFP. Taken together, our findings identify a new subunit of the mammalian Mediator and shed new light on the architecture of the mammalian Mediator complex.
Shigeo Sato; Chieri Tomomori-Sato; Charles A S Banks; Tari J Parmely; Irina Sorokina; Christopher S Brower; Ronald C Conaway; Joan Weliky Conaway
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-10-22
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  278     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-08     Completed Date:  2004-01-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  49671-4     Citation Subset:  IM    
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
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MeSH Terms
Amino Acid Sequence
Cell Line
Cell Nucleus / metabolism
DNA-Binding Proteins / chemistry,  genetics
Drosophila Proteins / chemistry*,  genetics
Drosophila melanogaster / metabolism*
Glutathione Transferase / metabolism
Hela Cells
Herpes Simplex Virus Protein Vmw65 / chemistry
Mass Spectrometry
Mediator Complex
Molecular Sequence Data
Open Reading Frames
Protein Binding
Protein Structure, Tertiary
RNA Polymerase II / metabolism
Recombinant Proteins / metabolism
Sequence Homology, Amino Acid
Transcription Factors / chemistry*,  genetics,  metabolism*
Transcriptional Activation*
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/DSX protein, Drosophila; 0/Drosophila Proteins; 0/Herpes Simplex Virus Protein Vmw65; 0/MED8 protein, human; 0/Mediator Complex; 0/Recombinant Proteins; 0/Transcription Factors; 0/intersex protein, Drosophila; 0/intersex protein, human; EC Transferase; EC 2.7.7.-/RNA Polymerase II

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