| A male murine model of partial bladder outlet obstruction reveals changes in detrusor morphology, contractility and Myosin isoform expression. | |
| | |
MedLine Citation:
|
PMID: 15371884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Mice with gene deletion or targeted over expression are important for understanding the remodeling that follows partial bladder outlet obstruction (PBOO). This condition predominates in males. We produced PBOO in male mice and now report the physiological, histological and molecular consequences. MATERIALS AND METHODS: Male C57bl/6 mice were surgically obstructed or subjected to sham surgery and unoperated mice served as controls. Four weeks later the bladders were excised and their function was assessed with in vitro whole bladder cystometry. The optimum volume for pressure generation was determined and isometric pressures were measured for field stimulation and depolarization with KCl. Bladder hypertrophy was classified as severe-bladder mass greater than 50 mg or mild-bladder mass less than 50 mg. The percent muscle fraction was determined by histological analyses. The expression of C-terminal (SM1 and SM2) and N-terminal (SM-B and SM-A) isoforms of myosin heavy chain was analyzed by reverse transcriptase-polymerase chain reaction. RESULTS: Severely hypertrophied bladders had larger optimum volume (p >0.001) and generated less pressure in response to field stimulation (p >0.001) and KCl (p >0.01) with a slower rate of pressure generation than controls or sham operated mice. Increased SM1-to-SM2 and SM-A-to-SM-B ratios were noted in severely obstructed bladders relative to controls or sham operated mice (p <0.05). The muscle fraction decreased slightly in the severely hypertrophied group (p not significant). CONCLUSIONS: Our male mouse model of PBOO demonstrates an increase in bladder mass, larger capacity and significantly decreased pressure generation in the in vitro whole bladder model. Obstruction induced increases in the expression of C-terminal (SM1) and N-terminal (SM-A) myosin heavy chain isoforms. |
| | |
Authors:
|
J Christopher Austin; Samuel K Chacko; Michael DiSanto; Douglas A Canning; Stephen A Zderic |
Related Documents
:
|
2008774 - Urodynamic evaluation of lower urinary tract function in cats after perineal urethrosto... 7706574 - Intra-abdominal pressure measurement using a modified nasogastric tube: description and... 14713804 - Urodynamically defined stress urinary incontinence and bladder outlet obstruction coexi... 11488724 - Acute urinary retention: defining the need and timing for pressure-flow studies. 15800124 - Relative glomerular hyperfiltration in primary aldosteronism. 15260084 - Effect of oral tizanidine on local-anesthetic infiltration pain during epidural cathete... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of urology Volume: 172 ISSN: 0022-5347 ISO Abbreviation: J. Urol. Publication Date: 2004 Oct |
Date Detail:
|
Created Date: 2004-09-16 Completed Date: 2004-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376374 Medline TA: J Urol Country: United States |
Other Details:
|
Languages: eng Pagination: 1524-8 Citation Subset: AIM; IM |
Affiliation:
|
Division of Urology Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Disease Models, Animal Gene Expression Regulation, Enzymologic Hypertrophy Isoenzymes / analysis, genetics Isometric Contraction / genetics, physiology* Male Mice Mice, Inbred C57BL Muscle Hypertonia / genetics, pathology* Muscle, Smooth / pathology Myosin Heavy Chains / analysis*, genetics Reverse Transcriptase Polymerase Chain Reaction Urinary Bladder / pathology Urinary Bladder Neck Obstruction / genetics, pathology* |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; 0/Myosin Heavy Chains |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of cholinergic drugs on ureteral function in anesthetized dogs.
Next Document: Intravesical botulinum toxin a administration produces analgesia against acetic acid induced bladder...