| A major substrate for MPF: cDNA cloning and expression of polypeptide chain elongation factor 1 gamma from goldfish (Carassius auratus). | |
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MedLine Citation:
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PMID: 12180134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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One of the eukaryotic polypeptide chain elongation factors, EF-1 beta gamma delta complex, is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1 alpha. In the complex, EF-1 gamma has been reported to be a major substrate for maturation promoting factor (MPF). Here, we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, EF-1 gamma from the goldfish ovary. The cloned cDNA was 1490 bp in length and encoded 442 amino acids. The deduced amino acid sequence was highly homologous to EF-1 gamma from other species. Although, the phosphorylation site identified in Xenopus EF-1 gamma was not conserved in the goldfish homologue, phosphorylation analysis showed that the goldfish EF-1 gamma was phosphorylated by MPF. We concluded that EF-1 gamma is a substrate for MPF during oocyte maturation in goldfish. |
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Authors:
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Mika Tokumoto; Yoshitaka Nagahama; Toshinobu Tokumoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: DNA sequence : the journal of DNA sequencing and mapping Volume: 13 ISSN: 1042-5179 ISO Abbreviation: DNA Seq. Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-08-15 Completed Date: 2002-12-30 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9107800 Medline TA: DNA Seq Country: England |
Other Details:
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Languages: eng Pagination: 27-31 Citation Subset: IM |
Affiliation:
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Faculty of Science, Department of Biology and Geosciences, Shizuoka University, Shizuoka 422-8529, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB056105 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Goldfish / genetics, metabolism Humans Maturation-Promoting Factor / metabolism* Molecular Sequence Data Peptide Elongation Factor 1 / genetics, metabolism* Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Peptide Elongation Factor 1; EC 2.7.11.22/Maturation-Promoting Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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