Document Detail

A major role for phospholipase A2 in antigen-induced arachidonic acid release in rat mast cells.
MedLine Citation:
PMID:  3120703     Owner:  NLM     Status:  MEDLINE    
Cross-linking of IgE receptors by antigen stimulation leads to histamine release and arachidonic acid release in rat peritoneal mast cells. Investigators have reported a diverse distribution of [3H]arachidonate that is dependent on labelling conditions. Mast cells from rat peritoneal cavity were labelled with [3H]arachidonic acid for different periods of time at either 30 or 37 degrees C. Optimum labelling was found to be after 4 h incubation with [3H]arachidonate at 30 degrees C, as judged by cell viability (Trypan Blue uptake), responsiveness (histamine release) and distribution of radioactivity. Alterations in 3H-radioactivity distribution in mast cells labelled to equilibrium were examined on stimulation with antigen (2,4-dinitrophenyl-conjugated Ascaris suum extract). The results indicated that [3H]arachidonic acid was lost mainly from phosphatidylcholine and, to a lesser extent, from phosphatidylinositol. A transient appearance of radiolabelled phosphatidic acid and diacylglycerol indicated phosphatidylinositol hydrolysis by phospholipase C. Pretreatment with a phospholipase A2 inhibitor, mepacrine, substantially prevented the antigen-induced liberation of [3H]arachidonic acid from phosphatidylcholine. It can be thus concluded that, in the release of arachidonic acid by antigen-stimulated mast cells, the phospholipase A2 pathway, in which phosphatidylcholine is hydrolysed, serves as the major one, the phospholipase C/diacylglycerol lipase pathway playing only a minor role.
K Yamada; Y Okano; K Miura; Y Nozawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  247     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1988-01-14     Completed Date:  1988-01-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  95-9     Citation Subset:  IM    
Department of Biochemistry, Gifu University School of Medicine, Japan.
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MeSH Terms
Antigens / pharmacology*
Antigens, Helminth
Arachidonic Acid
Arachidonic Acids / pharmacokinetics*
Fatty Acids, Nonesterified / metabolism
Lipid Metabolism
Mast Cells / drug effects,  metabolism*
Phospholipases / metabolism*
Phospholipases A / metabolism*
Phospholipases A2
Quinacrine / pharmacology
Rats, Inbred Strains
Reg. No./Substance:
0/Antigens; 0/Antigens, Helminth; 0/Arachidonic Acids; 0/Fatty Acids, Nonesterified; 506-32-1/Arachidonic Acid; 83-89-6/Quinacrine; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC A2

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