| The major myristoylated PKC substrate (MARCKS) is involved in cell spreading, tyrosine phosphorylation of paxillin, and focal contact formation. | |
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MedLine Citation:
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PMID: 9426227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression of the myristoylated PKC substrate MARCKS is reduced in tumor-derived choroidal melanoma cells (OCM-1). We transfected the OCM-1 cells with MARCKS cDNA and we selected clones with stable overexpression of the protein. Tyrosine phosphorylation of paxillin, a biochemical marker of focal contact formation, was conserved upon serum starvation when MARCKS was overexpressed, while it was almost abolished in the control cells. Immunofluorescent labelling of paxillin and vinculin, another component of focal contact, revealed that these structures were conserved upon serum starvation when MARCKS was overexpressed but not in the control cells. Furthermore, the cell morphology was affected by the ectopic expression of MARCKS, leading to increased spreading and formation of membrane processes. These data suggest the involvement of MARCKS in cell spreading and focal contact formation. |
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Authors:
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S Manenti; F Malecaze; J M Darbon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 419 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-01-26 Completed Date: 1998-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 95-8 Citation Subset: IM |
Affiliation:
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Institut National de la Santé et de la Recherche Médicale, Institut Fédératif de Recherche 30, Hopital Purpan, Toulouse, France. manenti@purpan.inserm.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion
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physiology* Cell Adhesion Molecules / analysis, metabolism* Cell Size Choroid Neoplasms Cytoskeletal Proteins / analysis, metabolism* Humans Intracellular Signaling Peptides and Proteins* Melanoma Membrane Proteins* Paxillin Phosphoproteins / analysis, metabolism* Phosphorylation Proteins / genetics, physiology* Transfection Tumor Cells, Cultured Tyrosine / metabolism* Vinculin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Cell Adhesion Molecules; 0/Cytoskeletal Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/PXN protein, human; 0/Paxillin; 0/Phosphoproteins; 0/Proteins; 125267-21-2/myristoylated alanine-rich C kinase substrate; 125361-02-6/Vinculin; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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