Document Detail

The major myristoylated PKC substrate (MARCKS) is involved in cell spreading, tyrosine phosphorylation of paxillin, and focal contact formation.
MedLine Citation:
PMID:  9426227     Owner:  NLM     Status:  MEDLINE    
The expression of the myristoylated PKC substrate MARCKS is reduced in tumor-derived choroidal melanoma cells (OCM-1). We transfected the OCM-1 cells with MARCKS cDNA and we selected clones with stable overexpression of the protein. Tyrosine phosphorylation of paxillin, a biochemical marker of focal contact formation, was conserved upon serum starvation when MARCKS was overexpressed, while it was almost abolished in the control cells. Immunofluorescent labelling of paxillin and vinculin, another component of focal contact, revealed that these structures were conserved upon serum starvation when MARCKS was overexpressed but not in the control cells. Furthermore, the cell morphology was affected by the ectopic expression of MARCKS, leading to increased spreading and formation of membrane processes. These data suggest the involvement of MARCKS in cell spreading and focal contact formation.
S Manenti; F Malecaze; J M Darbon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  419     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-26     Completed Date:  1998-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  95-8     Citation Subset:  IM    
Institut National de la Santé et de la Recherche Médicale, Institut Fédératif de Recherche 30, Hopital Purpan, Toulouse, France.
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MeSH Terms
Cell Adhesion / physiology*
Cell Adhesion Molecules / analysis,  metabolism*
Cell Size
Choroid Neoplasms
Cytoskeletal Proteins / analysis,  metabolism*
Intracellular Signaling Peptides and Proteins*
Membrane Proteins*
Phosphoproteins / analysis,  metabolism*
Proteins / genetics,  physiology*
Tumor Cells, Cultured
Tyrosine / metabolism*
Vinculin / analysis
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Cytoskeletal Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/PXN protein, human; 0/Paxillin; 0/Phosphoproteins; 0/Proteins; 125267-21-2/myristoylated alanine-rich C kinase substrate; 125361-02-6/Vinculin; 55520-40-6/Tyrosine

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