| The major and minor wall teichoic acids prevent the sidewall localization of vegetative DL-endopeptidase LytF in Bacillus subtilis. | |
| | |
MedLine Citation:
|
PMID: 18761696 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell separation in Bacillus subtilis depends on specific activities of DL-endopeptidases CwlS, LytF and LytE. Immunofluorescence microscopy (IFM) indicated that the localization of LytF depended on its N-terminal LysM domain. In addition, we revealed that the LysM domain efficiently binds to peptidoglycan (PG) prepared by chemically removing wall teichoic acids (WTAs) from the B. subtilis cell wall. Moreover, increasing amounts of the LysM domain bound to TagB- or TagO-depleted cell walls. These results strongly suggested that the LysM domain specifically binds to PG, and that the binding may be prevented by WTAs. IFM with TagB-, TagF- or TagO-reduced cells indicated that LytF-6xFLAG was observed not only at cell separation site and poles but also as a helical pattern along the sidewall. Moreover, we found that LytF was localizable on the whole cell surface in TagB-, TagF- or TagO-depleted cells. These results strongly suggest that WTAs inhibit the sidewall localization of LytF. Furthermore, the helical LytF localization was observed on the lateral cell surface in MreB-depleted cells, suggesting that cell wall modification by WTAs along the sidewall might be governed by an actin-like cytoskeleton homologue, MreB. |
| | |
Authors:
|
Hiroki Yamamoto; Yukiko Miyake; Miharu Hisaoka; Shin-Ichirou Kurosawa; Junichi Sekiguchi |
Related Documents
:
|
3163676 - Possible role of a proteinase in endosporulation of coccidioides immitis. 12634046 - A mechanics model for the compression of plant and vegetative tissues. 6683876 - A zygomycotic infection in captive snakes. 16668166 - Auxin-enhanced glucan autohydrolysis in maize coleoptile cell walls. 8810346 - Cell-surface cytokeratin 8 is the major plasminogen receptor on breast cancer cells and... 7306546 - Control of passive permeability of chinese hamster ovary cells by external and intracel... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-22 |
Journal Detail:
|
Title: Molecular microbiology Volume: 70 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2008 Oct |
Date Detail:
|
Created Date: 2008-10-17 Completed Date: 2008-12-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 297-310 Citation Subset: IM |
Affiliation:
|
Experimental Farm, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
enzymology* Bacterial Proteins / genetics, metabolism Blotting, Western Cell Wall / chemistry*, enzymology* Electrophoresis, Polyacrylamide Gel Endopeptidases / genetics, metabolism* Microscopy, Fluorescence Microscopy, Immunoelectron Peptidoglycan / metabolism Protein Binding Protein Structure, Tertiary Teichoic Acids / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Peptidoglycan; 0/Teichoic Acids; EC 3.4.-/Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the...
Next Document: Sortase-mediated assembly and surface topology of adhesive pneumococcal pili.