Document Detail

The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning.
MedLine Citation:
PMID:  6207938     Owner:  NLM     Status:  MEDLINE    
Synthetic oligonucleotide probes have been used to identify two overlapping cDNA clones that represent the entire coding region of the mRNA for the major intrinsic protein (MIP) of bovine lens cell membrane. Hybridization studies indicate that bovine MIP is encoded by a single-copy gene. The cDNA hybridizes to the rat genome, but MIP mRNA is not detected in rat liver. Analysis of the deduced amino acid sequence provides support for the potential role of MIP as a junctional protein. The structure predicted for MIP suggests that it traverses the lipid bilayer six times with both carboxy and amino termini on the cytoplasmic side, and that it has at least one amphiphilic transmembrane segment, as expected if the protein were to participate in the formation of an aqueous channel.
M B Gorin; S B Yancey; J Cline; J P Revel; J Horwitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  39     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-12     Completed Date:  1984-12-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  49-59     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Membrane / metabolism
Cloning, Molecular*
DNA / metabolism*
DNA Restriction Enzymes
Eye Proteins / genetics*
Lens, Crystalline / cytology,  metabolism*
Membrane Glycoproteins*
Nucleic Acid Conformation
Poly A / genetics
RNA / genetics
RNA, Messenger / genetics
Grant Support
Reg. No./Substance:
0/Aquaporins; 0/Eye Proteins; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/aquaporin 0; 24937-83-5/Poly A; 63231-63-0/RNA; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

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