Document Detail


The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning.
MedLine Citation:
PMID:  6207938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Synthetic oligonucleotide probes have been used to identify two overlapping cDNA clones that represent the entire coding region of the mRNA for the major intrinsic protein (MIP) of bovine lens cell membrane. Hybridization studies indicate that bovine MIP is encoded by a single-copy gene. The cDNA hybridizes to the rat genome, but MIP mRNA is not detected in rat liver. Analysis of the deduced amino acid sequence provides support for the potential role of MIP as a junctional protein. The structure predicted for MIP suggests that it traverses the lipid bilayer six times with both carboxy and amino termini on the cytoplasmic side, and that it has at least one amphiphilic transmembrane segment, as expected if the protein were to participate in the formation of an aqueous channel.
Authors:
M B Gorin; S B Yancey; J Cline; J P Revel; J Horwitz
Related Documents :
2271648 - Characterization of two new members of the pregnancy-specific beta 1-glycoprotein famil...
10721728 - Ermap, a gene coding for a novel erythroid specific adhesion/receptor membrane protein.
2322278 - Functional expression of human leukocyte elastase (hle)/medullasin in eukaryotic cells.
9195948 - Cloning, in vitro expression, and functional characterization of a novel human cc chemo...
1640828 - Membrane-associated gtpases in bacteria.
22160908 - Yeasts as a tool for heterologous gene expression.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  39     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-12     Completed Date:  1984-12-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  49-59     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/K02818
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aquaporins
Base Sequence
Cattle
Cell Membrane / metabolism
Cloning, Molecular*
DNA / metabolism*
DNA Restriction Enzymes
Eye Proteins / genetics*
Genes*
Lens, Crystalline / cytology,  metabolism*
Membrane Glycoproteins*
Nucleic Acid Conformation
Poly A / genetics
RNA / genetics
RNA, Messenger / genetics
Grant Support
ID/Acronym/Agency:
EY07026/EY/NEI NIH HHS; GM 06965/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Aquaporins; 0/Eye Proteins; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/aquaporin 0; 24937-83-5/Poly A; 63231-63-0/RNA; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A homeo-box-containing gene expressed during oogenesis in Xenopus.
Next Document:  Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells d...