Document Detail


The maize transposable element Ac induces recombination between the donor site and an homologous ectopic sequence.
MedLine Citation:
PMID:  9215915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prominent repair mechanism of DNA double-strand breaks formed upon excision of the maize Ac transposable element is via nonhomologous end joining. In this work we have studied the role of homologous recombination as an additional repair pathway. To this end, we developed an assay whereby beta-Glucuronidase (GUS) activity is restored upon recombination between two homologous ectopic (nonallelic) sequences in transgenic tobacco plants. One of the recombination partners carried a deletion at the 5' end of GUS and an Ac or a Ds element inserted at the deletion site. The other partner carried an intact 5' end of the GUS open reading frame and had a deletion at the 3' end of the gene. Based on GUS reactivation data, we found that the excision of Ac induced recombination between ectopic sequences by at least two orders of magnitude. Recombination events, visualized by blue staining, were detected in seedlings, in pollen and in protoplasts. DNA fragments corresponding to recombination events were recovered exclusively in crosses with Ac-carrying plants, providing physical evidence for Ac-induced ectopic recombination. The occurrence of ectopic recombination following double-strand breaks is a potentially important factor in plant genome evolution.
Authors:
G Shalev; A A Levy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetics     Volume:  146     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-09-18     Completed Date:  1997-09-18     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1143-51     Citation Subset:  IM    
Affiliation:
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA Transposable Elements*
DNA, Plant
Genes, Plant*
Molecular Sequence Data
Recombination, Genetic*
Zea mays / genetics*
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; 0/DNA, Plant
Comments/Corrections

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